Analysis of dominant enhancers and suppressors of activated Notch in Drosophila.
Analysis of dominant enhancers and suppressors of activated Notch in Drosophila.
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果蝇中激活Notch的显性增强子和抑制子的分析。
DOI：
10.1093/genetics/144.3.1127
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Artavanis-Tsakonas,S
中科院分区：
文献类型：
--
作者：
Verheyen,EM;Purcell,KJ;Fortini,ME;Artavanis-Tsakonas,S
Notch 受体控制果蝇整个发育过程中的细胞命运决定。这种蛋白质的截短、配体独立形式延迟或阻止分化。我们之前已经表明，在七个增强子/启动子的控制下受体胞内结构域的表达会在成年果蝇中诱导粗糙的眼睛表型。对由此产生的细胞转化的分析表明，这种形式的 Notch 充当组成型激活的受体。为了鉴定与Notch相互作用的基因产物，进行了第二位点诱变筛选，以分离由这些激活的Notch分子表达引起的眼睛表型的增强子和抑制子。我们筛选了 137,000 只诱变果蝇并恢复了 290 个显性修饰因子。先前鉴定的基因的许多新等位基因被分离出来，定义可能在 Notch 信号通路中发挥作用的新位点的突变也是如此。我们讨论了有关 Notch 受体信号传导和果蝇眼睛发育的已知特征的数据。
The Notch receptor controls cell fate decisions throughout Drosophila development. Truncated, ligand-independent forms of this protein delay or block differentiation. We have previously shown that expression of the intracellular domain of the receptor under the control of thesevenlessenhancer/promoter induces a rough eye phenotype in the adult fly. Analysis of the resultant cellular transformations suggested that this form of Notch acts as a constitutively activated receptor. To identify gene products that interact withNotch, a second-site mutagenesis screen was performed to isolate enhancers and suppressors of the eye phenotype caused by expression of these activated Notch molecules. We screened 137,000 mutagenized flies and recovered 290 dominant modifiers. Many new alleles of previously identified genes were isolated, as were mutations defining novel loci that may function in the Notch signaling pathway. We discuss the data with respect to known features of Notch receptor signaling and Drosophila eye development.