Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P
Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P
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冠冰草染色体 2P 中新型白粉病抗性基因的定位。
DOI：
10.1007/s00122-016-2797-9
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发表时间：
2017-01-01
影响因子：
5.4
通讯作者：
Li, Lihui
中科院分区：
文献类型：
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作者：
Li, Huanhuan;Jiang, Bo;Li, Lihui
首次构建了冰草（Agropyron cristatum（L.）gaertn. (2n= 28，PPPP）是普通小麦的野生近缘种，对白粉病高度抗性。以往的研究表明，小麦-A. cristatum 2P二体异附加系II-9 - 3对白粉病表现高抗，抗性来源于A.冠突2P染色体。为了利用小麦抗白粉病基因并进行物理定位，对15个小麦-A。cristatum 2P易位系和3个A.利用Co-60-γ射线照射从II-9 - 3获得的具有不同染色体片段大小的cristatum 2P缺失系，使用细胞遗传学和分子标记分析进行表征。a.易位系中的cristatum 2P染色体片段分别易位到1A、4A、5A、6A、7A、1B、2B、3B、7B、3D、4D和6D等不同的小麦染色体上。用82个STS标记构建了2P染色体物理图谱，其中2PS上有9个bin，34个标记; 2PL上有8个bin，48个标记。7个小麦-A.采用自交法培育了2PT-3、2PT-4、2PT-5、2PT-6、2PT-8、2PT-9和2PT-10等7个冠状花序2P易位系，并进行了白粉病检测和2P特异STS标记基因型分析。结果表明，抗白粉病基因定位于2PL bin FL 0.66 - 0.86，在该bin中鉴定出19个2P特异标记。此外，还获得了2个新的抗白粉病易位系2PT-4和2PT-5。新育成的抗白粉病小麦新品系及其紧密连锁的分子标记将为小麦抗病育种提供重要的参考。
A physical map of Agropyron cristatum 2P chromosome was constructed for the first time and the novel powdery mildew resistance gene(s) from chromosome 2P was(were) also mapped.Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, is highly resistant to powdery mildew. Previous studies showed that wheat-A. cristatum 2P disomic addition line II-9-3 displayed high resistance to powdery mildew, and the resistance was attributable to A. cristatum chromosome 2P. To utilize and physically map the powdery mildew resistance gene(s), 15 wheat-A. cristatum 2P translocation lines and three A. cristatum 2P deletion lines with different chromosomal segment sizes, obtained from II-9-3 using Co-60-gamma ray irradiation, were characterized using cytogenetic and molecular marker analysis. A. cristatum 2P chromosomal segments in the translocations were translocated to different wheat chromosomes, including 1A, 4A, 5A, 6A, 7A, 1B, 2B, 3B, 7B, 3D, 4D, and 6D. A physical map of the 2P chromosome was constructed with 82 STS markers, consisting of nine bins with 34 markers on 2PS and eight bins with 48 markers on 2PL. The BC1F2 populations of seven wheat-A. cristatum 2P translocation lines (2PT-3, 2PT-4, 2PT-5, 2PT-6, 2PT-8, 2PT-9, and 2PT-10) were developed by self-pollination, tested with powdery mildew and genotyped with 2P-specific STS markers. From these results, the gene(s) conferring powdery mildew resistance was(were) located on 2PL bin FL 0.66-0.86 and 19 2P-specific markers were identified in this bin. Moreover, two new powdery mildew-resistant translocation lines (2PT-4 and 2PT-5) with small 2PL chromosome segments were obtained. The newly developed wheat lines with powdery mildew resistance and the closely linked molecular markers will be valuable for wheat disease breeding in the future.