Identification of stem-like cells in head and neck cancer cell lines.
Identification of stem-like cells in head and neck cancer cell lines.
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发表时间：
2010-06
影响因子：
2
通讯作者：
Guang-wei Sun;M. Fujii;A. Sonoda;Y. Tokumaru;T. Matsunaga;Noboru Habu
中科院分区：
文献类型：
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作者：
Guang-wei Sun;M. Fujii;A. Sonoda;Y. Tokumaru;T. Matsunaga;Noboru Habu
本研究调查了在已建立的头颈部鳞状细胞癌（HNSCC）细胞系HSC 3和HSC 4中干细胞样细胞的存在。流式细胞术分析证实，在HSC 4细胞中存在侧群（SP）细胞（不包括Hoechst 33342）（0.37+/-0.06%），但在HSC 3细胞中不存在利血平敏感性细胞。分选后，SP细胞在培养物中产生SP和主要群体（MP）细胞，而MP细胞仅产生MP细胞。SP细胞干细胞标志物的表达高于MP细胞。提示头颈部鳞状细胞癌中存在癌干细胞样细胞。
This study investigated the existence of stem-like cells in established head and neck squamous cell carcinoma (HNSCC) lines, HSC3 and HSC4. Flow cytometric analysis confirmed the presence of side population (SP) cells excluding Hoechst 33342 in HSC4 cells (0.37+/-0.06%) but not HSC3 cells in a reserpine-sensitive manner. After sorting, the SP cells generated both SP and main population (MP) cells in culture while MP cells generated MP cells only. Higher expression of stem cell markers was detected in SP than in MP cells. These results suggest that cancer stem-like cells exist in head and neck squamous cell carcinoma.