Selectivity comparisons of monolithic silica capillary columns modified with poly(octadecyl methacrylate) and octadecyl moieties for halogenated compounds in reversed-phase liquid chromatography.
Selectivity comparisons of monolithic silica capillary columns modified with poly(octadecyl methacrylate) and octadecyl moieties for halogenated compounds in reversed-phase liquid chromatography.
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DOI：
10.1016/j.chroma.2009.06.030
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
Wasura Soonthorntantikul;N. Leepipatpiboon;T. Ikegami;N. Tanaka;T. Nhujak
中科院分区：
文献类型：
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作者：
Wasura Soonthorntantikul;N. Leepipatpiboon;T. Ikegami;N. Tanaka;T. Nhujak
采用聚甲基丙烯酸十八烷基（ODM）和十八烷基（ODS）修饰的C18硅胶整体毛细管柱，比较了反相高效液相色谱中卤化化合物的固定相选择性。在甲醇/水和乙腈/水流动相中，观察到卤化苯在ODM上的优先保留。在ODM和ODS的选择性比较中，在ODM色谱柱上的卤代化合物对烷基苯的选择性更高，而在ODM和ODS色谱柱上的同源系列烷基苯的选择性相似。这些数据可以用ODM聚合物包覆柱上更密集的十八烷基以及ODM侧链上羰基的贡献所产生的更大的分散相互作用来解释。对于二卤代苯（邻位、间位、对位）的位置异构体分离，ODM柱也能更好地分离相邻的异构体，而ODS柱在同一流动相中不能完全分离。这些结果表明，ODM色谱柱可以作为ODS色谱柱的较好替代品，用于其它卤化化合物的分离。
Stationary phase selectivities for halogenated compounds in reversed-phase HPLC were compared using C18 monolithic silica capillary columns modified with poly(octadecyl methacrylate) (ODM) and octadecyl moieties (ODS). The preferential retention of halogenated benzenes on ODM was observed in methanol/water and acetonitrile/water mobile phases. In selectivity comparison of selected analytes on ODM and ODS, greater selectivities for halogenated compounds were obtained with respect to alkylbenzenes on an ODM column, while similar selectivities were observed with a homologous series of alkylbenzenes on ODM and ODS columns. These data can be explained by greater dispersive interactions by more densely packed octadecyl groups on the ODM polymer coated column together with the contribution of carbonyl groups in ODM side chains. For the positional isomeric separation of dihalogenated benzenes (ortho-, meta-, para-), the ODM column also provided better separation of these isomers for the adjacently eluted isomers that cannot be completely separated on an ODS column in the same mobile phase. These results imply that the ODM column can be used as a better alternative to the ODS column for the separation of other halogenated compounds.