Mass spectrometry-based methods for analysing the mitochondrial interactome in mammalian cells
Mass spectrometry-based methods for analysing the mitochondrial interactome in mammalian cells
复制标题
基于质谱分析哺乳动物细胞线粒体相互作用组的方法
DOI：
10.1093/jb/mvz090
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Kosako Hidetaka
中科院分区：
文献类型：
--
作者：
Koshiba Takumi;Kosako Hidetaka
蛋白质-蛋白质相互作用是发生在细胞间和细胞内水平上的基本生物过程。为了深入了解这些相互作用的各种复杂的细胞功能，有必要在生理条件下评估它们。各种蛋白质组学技术的最新进展使得研究活细胞中的蛋白质-蛋白质相互作用网络成为可能。邻近标记和化学交联的结合将极大地提高我们对难以制备的多蛋白质复合体的理解，例如细胞器结合的膜蛋白。在这篇综述中，我们描述了基于质谱学的蛋白质组学作图方法在阐明活细胞中细胞器结合的膜蛋白复合体方面的最新理解，重点介绍了线粒体亚细胞间的蛋白质-蛋白质相互作用。
Protein–protein interactions are essential biologic processes that occur at inter- and intracellular levels. To gain insight into the various complex cellular functions of these interactions, it is necessary to assess them under physiologic conditions. Recent advances in various proteomic technologies allow to investigate protein–protein interaction networks in living cells. The combination of proximity-dependent labelling and chemical cross-linking will greatly enhance our understanding of multi-protein complexes that are difficult to prepare, such as organelle-bound membrane proteins. In this review, we describe our current understanding of mass spectrometry-based proteomics mapping methods for elucidating organelle-bound membrane protein complexes in living cells, with a focus on protein–protein interactions in mitochondrial subcellular compartments.