B-cell CD25 expression in murine primary and secondary lymphoid tissue
B-cell CD25 expression in murine primary and secondary lymphoid tissue
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DOI：
10.1111/j.1365-3083.2006.01832.x
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发表时间：
2006-11-01
影响因子：
3.7
通讯作者：
Brisslert, M.
中科院分区：
文献类型：
--
作者：
Amu, S.;Gjertsson, I.;Brisslert, M.
B细胞类似于能够表达功能性IL-2受体的T细胞。IL-2R α链（CD25）阳性T细胞已被详细研究，但对CD25阳性B细胞知之甚少。本研究的目的是检测小鼠不同淋巴器官中表达CD25的B细胞的表型特性。对样品进行各种细胞表面标记染色，并用流式细胞术进行分析。我们发现骨髓中大约49%的B细胞、腹腔中16%的B细胞、脾脏中2%的B细胞和淋巴结中1%的B细胞表达CD25。相比之下，在血液或Peyer’s斑块中未发现表达CD25的B细胞。表型表征表明，脾脏、淋巴结和腹腔的CD25(+) B细胞表面表达的AA4.1、CD5、CD69、CD80、CD86、CD122、CD132、IgA、IgG和IgM均高于CD25(-) B细胞。脾脏和淋巴结CD25(+) B细胞IgD和IA-IE表达较低。在骨髓中，CD5、CD80、CD86、CD122、CD132、IgA、IgD和IgM的表达较低，而AA4.1、IgG和IA-IE在CD25(+) B细胞上的表达较CD25(-) B细胞升高。总之，我们的研究结果表明，表达CD25的B细胞在表型上明显不同于CD25阴性的B细胞。我们的研究结果表明，CD25(+) B细胞更倾向于有效的抗原呈递，并表现出更成熟的表型。
B cells are in analogy with T cells capable of expressing functional IL-2 receptors. IL-2R alpha-chain (CD25) positive T cells have been studied in detail but not much is known about CD25 positive B cells. The aim of this study was to examine the phenotypic properties of the CD25 expressing B cells collected from different lymphoid organs in mice. Samples were stained for various cell surface markers and analysed using flow cytometry. We found that approximately 49% of B cells in bone marrow, 16% in peritoneal cavity, 2% in spleen and 1% in lymph nodes express CD25. In contrast, CD25 expressing B cells were not found in the blood or in Peyer's patches. Phenotypic characterization showed that CD25(+) B cells in spleen, lymph nodes and peritoneal cavity have higher expression of AA4.1, CD5, CD69, CD80, CD86, CD122, CD132, IgA, IgG and IgM on their surface in comparison with CD25(-) B cells. In contrast, expression of IgD and IA-IE was lower on CD25(+) B cells in spleen and lymph nodes. In bone marrow, the expression of CD5, CD80, CD86, CD122, CD132, IgA, IgD and IgM was lower, while the expression of AA4.1, IgG and IA-IE was increased on CD25(+) B cells compared with CD25(-) B cells. In conclusion, our results indicate that B cells expressing CD25 are phenotypically distinctly different from those that are CD25 negative. Our findings suggest that CD25(+) B cells are more prone to efficient antigen presentation and display a more mature phenotype.