Preparation of 125 I-labelled synthetic human gastrin I for radioimmunoanalysis.
Preparation of 125 I-labelled synthetic human gastrin I for radioimmunoanalysis.
复制标题
用于放射免疫分析的 125 I 标记的合成人胃泌素 I 的制备。
DOI：
10.3109/00365517209080271
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发表时间：
1972
影响因子：
2.1
通讯作者：
J. Rehfeld
中科院分区：
文献类型：
--
作者：
F. Stadil;J. Rehfeld
本文报道了一种可重复的人工合成人胃泌素的标记方法。过量的激素用[125 I]NaI通过氯胺T法的改进进行碘化。在低浓度氯胺T（0.6 mmol/l）和低放射性水平（200 μCi）下进行反应。用AE-41纤维素柱进行梯度洗脱层析，将标记激素与未标记激素进行纯化和分离。合成的人胃泌素是异质的，但只需要一个纯化步骤。发现标记的制备物是均匀的，具有高比放射性（702 + 28 μCi/nmol，平均值± S.E.M.）。免疫反应性大大提高。示踪剂是稳定的，可雇用至少三个月，而胃泌素标记的氯胺T技术的常规修改可以使用只有三到四个星期。
AbstractA reproducible procedure for labelling of synthetic human gastrin has been developed. An excess of hormone was iodinated with [125I]NaI by a modification of the chloramine T method. The reaction was performed at a low concentration of chloramine T (0.6 mmol/1) and at a low level of radioactivity (200 μCi). Purification and separation of labelled from unlabelled hormone was carried out by gradient elution chromatography on an AE-41 cellulose column. Synthetic human gastrin was heterogeneous, but only one purification step was needed. The labelled preparation was found to be homogeneous and of high specific radioactivity (702 + 28 μCi/nmol, mean ± S.E.M.). The immunoreactivity was considerably improved. The tracer was stable and employable for at least three months, while gastrin labelled by conventional modifications of the chloramine T technique could be used for only three to four weeks.