Spiroplasma leucomae sp. nov., isolated in Poland from white satin moth (Leucoma salicis L.) larvae.
Spiroplasma leucomae sp. nov., isolated in Poland from white satin moth (Leucoma salicis L.) larvae.
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白色螺原体 sp. leucomae sp.
DOI：
10.1099/ijs.0.63685-0
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发表时间：
2005
影响因子：
2.8
通讯作者：
Gasparich,GailE
中科院分区：
文献类型：
--
作者：
Oduori,MariettaA;Lipa,JerzyJ;Gasparich,GailE
螺原体属菌株SMAT，分离自波兰的白色柳柳粉虱Leucoma salicisL.（鳞翅目：毒蛾科），血清学上与其他螺原体种、群或亚群不同。暗视野显微镜的细胞揭示了经典的螺旋形状和随后的透射电子显微镜显示细胞包围只有一个细胞膜（缺乏细胞壁）。SMAT菌株在30 °C的M1 D培养基中生长。 SMAT菌株能降解葡萄糖和精氨酸，但不水解尿素。DNA的G+C含量为24±1摩尔%，通过解链温度分析测定。 血清学分析显示，与两个螺旋体菌株277 F（螺旋体）有极弱的交叉反应性（仅在1：80稀释时呈阳性反应）。 组I-4）和LB-12（Spiroplasmasp. I-5组）。菌株SMAT（=ATCC BAA-521 T =NBRC 100392 T）被指定为新物种白瘤螺原体的模式菌株。nov.（柔膜门纲虫原体目螺原体科）。
Spiroplasmasp. strain SMAT, isolated in Poland from white satin moth larvae,Leucoma salicisL. (Lepidoptera: Lymantriidae), was serologically distinct from otherSpiroplasmaspecies, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a single cell membrane (lacking a cell wall). Growth of strain SMAToccurred in M1D medium at 30 °C. Strain SMATcatabolized both glucose and arginine, but did not hydrolyse urea. The G+C content of the DNA was 24±1 mol% as determined by melting temperature analysis. Serological analysis revealed a very weak cross-reactivity (positive reaction only up to a 1 : 80 dilution) with twoSpiroplasmastrains, 277F (Spiroplasmasp. group I-4) and LB-12 (Spiroplasmasp. group I-5). Strain SMAT(=ATCC BAA-521T=NBRC 100392T) is designated the type strain of a novel species,Spiroplasma leucomaesp. nov. (classMollicutes: orderEntomoplasmatales: familySpiroplasmataceae).