Light-/dark-induced changes in rhabdom structure in the retina of Octopus bimaculoides.
Light-/dark-induced changes in rhabdom structure in the retina of Octopus bimaculoides.
复制标题
光/暗诱导的双斑章鱼视网膜横纹结构的变化。
DOI：
10.1007/s004410050918
复制
发表时间：
1997
影响因子：
3.6
通讯作者：
Robles,LJ
中科院分区：
文献类型：
--
作者：
Torres,SC;Camacho,JL;Matsumoto,B;Kuramoto,RT;Robles,LJ
我们研究了Octopus bimaculoides视网膜光感受器外节的横纹束结构和丝状肌动蛋白的分布。统计分析被用来比较两种光照条件下和之间的横纹肌微绒毛和核心细胞质的相对横截面积。还制备了暗/光适应的杆状物用于共聚焦激光扫描显微镜，并用荧光标记的鬼笔环肽标记。结果表明，在黑暗和光适应的章鱼横纹肌的形态与横纹肌的横截面积增加，在黑暗中适应的视网膜和减少的光。照明条件之间的比较表明，一个avillar部分的感光细胞外节膜，突出的光适应视网膜，招募形成新的横纹肌微绒毛在暗适应的眼睛。微丝肌动蛋白与光适应视网膜中的卵丘膜相关，这可能表明肌动蛋白和其他微绒毛核心蛋白仍然与卵丘膜连接，以支持黑暗中快速微绒毛形成。光色素再分布也发生在光和暗适应视网膜，这项研究表明，这些变化必须协调与横纹肌微绒毛的同时击穿和改造。
We examined rhabdom structure and the distribution of filamentous actin in the photoreceptor outer segments of the retina ofOctopus bimaculoides.Animals were dark- or light-adapted, fixed, embedded and sectioned for light and electron microscopy. Statistical analyses were used to compare relative cross-sectional areas of rhabdom microvilli and core cytoplasm within and between the two lighting conditions. Dark-/light-adapted rhabdoms were also prepared for confocal laser scanning microscopy and labeled with fluorescence-tagged phalloidin. Results show differences in the morphology of dark- and light-adapted octopus rhabdoms with the cross-sectional areas of the rhabdoms increasing in dark-adapted retinas and diminishing in the light. Comparisons between the lighting conditions show that an avillar portion of the photoreceptor outer segment membrane, prominent in the light-adapted retina, is recruited to form new rhabdomere microvilli in dark-adapted eyes. Filamentous actin was associated with the avillar membrane in light-adapted retinas, which may indicate that actin and other microvillus core proteins remain linked to the avillar membrane to support rapid microvillus formation in the dark. Photopigment redistributions also occur in light- and dark-adapted retinas, and this study suggests that these changes must be coordinated with the simultaneous breakdown and reformation of the rhabdomere microvilli.