Purification of rat urinary kallikreins and their specific antibody.
Purification of rat urinary kallikreins and their specific antibody.
复制标题
大鼠尿激肽释放酶及其特异性抗体的纯化。
DOI：
10.1021/bi00708a012
复制
发表时间：
1974
期刊：
影响因子：
2.9
通讯作者：
J. Pierce
中科院分区：
文献类型：
--
作者：
K. Nustad;J. Pierce
摘要：通过加压透析、DEAE-Sephadex A-50柱层析、羟基磷灰石柱层析、电聚焦等方法，获得了4种具有生物活性和免疫同一性的大鼠尿激肽释放酶(BiB4)。用十二烷基硫酸钠-聚丙烯酰胺圆盘法测定激肽释放酶的相对分子质量分别为35,300，33,600，33,100和32,300
Kjell NustadJ and Jack V. Pierce* abstract: Four biologically active and immunologically identical rat urinary kallikreins (Bi—B4) have been obtained in highly purified form bypressure dialysis, chromatography on DEAE-Sephadex A-50 and hydroxylapatite columns, and electrofocusing. Molecular weights of 35,300, 33,600, 33,100, and 32,300 were estimated for kallikreins Bi, Bo, B: i, and B4, respectively, by sodium dodecyl sulfate polyacrylamide disc