The OsDHODH1 Gene is Involved in Salt and Drought Tolerance in Rice
The OsDHODH1 Gene is Involved in Salt and Drought Tolerance in Rice
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OsDHODH1 基因参与水稻的耐盐性和耐旱性
DOI：
10.1111/j.1744-7909.2009.00853.x
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发表时间：
2009-09-01
影响因子：
11.4
通讯作者：
Zhang, Hong-Sheng
中科院分区：
文献类型：
--
作者：
Liu, Wen-Ying;Wang, Mei-Mei;Zhang, Hong-Sheng
在本文中，我们确定和克隆OsDHODH 1编码一个假定的细胞溶质二氢乳清酸脱氢酶（DHODH）在水稻。OsDHODH1的表达分析表明，盐，干旱和外源脱落酸（阿坝）的上调，但不受寒冷。通过原核表达，我们测定了OsDHODH 1的酶活性，发现过量表达OsDHODH 1能显著提高大肠杆菌细胞对盐和渗透胁迫的耐受性。与野生型和OsDHODH1反义转基因植株相比，OsDHODH1基因在水稻中的过表达提高了DHODH活性，增强了植物对盐和干旱胁迫的耐受性。我们的研究结果首次揭示了胞质二氢乳清酸脱氢酶参与植物的胁迫反应，OsDHODH 1可用于工程作物，增强耐盐性和耐旱性。
In the present paper, we identified and cloned OsDHODH1 encoding a putative cytosolic dihydroorotate dehydrogenase (DHODH) in rice. Expression analysis indicated that OsDHODH1 is upregulated by salt, drought and exogenous abscisic acid (ABA), but not by cold. By prokaryotic expression, we determined the enzymatic activity of OsDHODH1 and found that overproduction of OsDHODH1 significantly improved the tolerance of Escherichia coli cells to salt and osmotic stresses. Overexpression of the OsDHODH1 gene in rice increased the DHODH activity and enhanced plant tolerance to salt and drought stresses as compared with wild type and OsDHODH1-antisense transgenic plants. Our findings reveal, for the first time, that cytosolic dihydroorotate dehydrogenase is involved in plant stress response and that OsDHODH1 could be used in engineering crop plants with enhanced tolerance to salt and drought.