A tumour-associated DEAD-box protein, rck/p54 exhibits RNA unwinding activity toward c-myc RNAs in vitro
A tumour-associated DEAD-box protein, rck/p54 exhibits RNA unwinding activity toward c-myc RNAs in vitro
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DOI：
10.1046/j.1365-2443.2003.00665.x
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发表时间：
2003-08-01
期刊：
影响因子：
2.1
通讯作者：
Usukura, J
中科院分区：
文献类型：
--
作者：
Akao, Y;Yoshida, H;Usukura, J
背景：rck/p54蛋白由473个氨基酸组成，属于DEAD-box/推定RNA解旋酶蛋白家族。DEAD盒蛋白参与了从蛋白质合成和核糖体生物合成的起始到通过修饰RNA结构进行的前体mRNA剪接的各种细胞过程。结果：形态学研究和表面等离子体共振分析结果表明，rck/p54蛋白与c-myc RNA转录物（RNA）特异性结合，并在体外ATP存在下表现出对c-myc RNA的解旋活性。实验结果表明，rck/p54缺失突变体的N端289个氨基酸残基，其C端184个氨基酸残基的缺失与RNA解旋活性有关。结论：rck/p54在细胞内可能通过重组mRNA而在翻译起始中起重要作用，并参与肿瘤的发生。
Background: The rck/p54 protein of 473 amino acids belongs to the family of DEAD-box/putative RNA helicase proteins. DEAD-box proteins have been implicated in a wide variety of cellular processes ranging from the initiation of protein synthesis and ribosome biosynthesis to premRNA splicing by means of modifying the RNA structure. Our previous data suggested that rck/p54 positively affected the translation initiation of c-myc mRNA.Results: The data obtained from morphological studies and surface plasmon resonance assays clearly indicated that the protein specifically bound to c-myc RNA transcripts (RNAs) and exhibited RNA unwinding activity toward c-myc RNAs in the presence of ATP in vitro . Experiments using a deletion mutant of rck/p54 retaining only its N-terminal 289 amino acids demonstrated that the deleted C-terminal 184 amino acid domain is involved in the RNA unwinding activity.Conclusion: These findings strongly suggest that rck/p54 may play an important role in translation initiation by restructuring mRNAs even in the cell and contribute to carcinogenesis.