Changes in the patterns of collagens and fibronectin during limb-bud chondrogenesis.
Changes in the patterns of collagens and fibronectin during limb-bud chondrogenesis.
复制标题
肢芽软骨形成过程中胶原蛋白和纤连蛋白模式的变化。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
S. Fischer
中科院分区：
文献类型：
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作者：
W. Dessau;H. von der Mark;K. von der Mark;S. Fischer
用免疫荧光法研究了鸡胚后肢芽软骨发育模型中纤维连接蛋白和I型、II型胶原蛋白的分布和出现顺序。纤连蛋白和I型胶原蛋白均匀分布在整个间充质的细胞间隙之前，冷凝的核心间充质的肢体原基和软骨胚基的形成。随着冷凝过程的开始，纤连蛋白和I型胶原蛋白在软骨胚基中与周围的松散间充质相比似乎增加，在软骨分化时达到最大密度。后一个过程的特点是软骨胚基中出现II型胶原。随着软骨分化的进展，I型胶原蛋白逐渐被II型胶原蛋白取代;纤维连接蛋白消失，完全不存在于成熟的软骨中。I型胶原蛋白和纤连蛋白的短暂出现表明在软骨形成中细胞-基质或细胞-细胞相互作用中的暂时作用，因为已经显示（a）I型胶原蛋白底物刺激肢芽间充质细胞培养物中的细胞增殖和软骨分化;（B）纤连蛋白介导细胞与胶原蛋白底物的附着;和（c）纤连蛋白直接参与软骨细胞培养物中的细胞相互作用。
The distribution and sequence of appearance of fibronectin and of type-I and type-II collagen in the developing cartilage models of embryonic chick hind-limb buds was studied by immunofluorescence, using specific antibodies directed against these proteins. Fibronectin and type-I collagen are evenly distributed throughout the intercellular space of the mesenchyme prior to condensation of core mesenchyme of the limb anlage and formation of the cartilage blastema. With the onset of the condensation process fibronectin and type-I collagen appear to increase in the cartilage blastema compared to the surrounding loose mesenchyme, reaching a maximal density at the time of cartilage differentiation. The latter process is marked by the appearance of type-II collagen in the cartilage blastema. As cartilage differentiation progresses, type-I collagen is gradually replaced by type-II collagen; fibronectin disappears and is completely absent from mature cartilage. The transient appearance of type-I collagen and fibronectin suggests a temporal role in cell-matrix or cell-cell interactions in chondrogenesis, since it had been shown that (a) type-I collagen substrates stimulate cell proliferation and cartilage differentiation in limb-bud mesenchyme cell cultures; (b) fibronectin mediates attachment of cells to collagen substrates; and (c) fibronectin is directly involved in cellular interactions in chondrocyte cultures.