Vitamin B6 Vitamer Concentrations in Cerebrospinal Fluid Differ Between Preterm and Term Newborn Infants
Vitamin B6 Vitamer Concentrations in Cerebrospinal Fluid Differ Between Preterm and Term Newborn Infants
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DOI：
10.1542/peds.2011-3751
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发表时间：
2012-07-01
期刊：
影响因子：
8
通讯作者：
Verhoeven-Duif, Nanda M.
中科院分区：
文献类型：
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作者：
Albersen, Monique;Groenendaal, Floris;Verhoeven-Duif, Nanda M.
背景与目的：维生素B-6在大脑发育和功能中起着关键作用。早产儿和足月新生儿之间维生素B-6稳态的差异已有报道。方法：采用超高效液相色谱-串联质谱法测定36例新生儿（早产儿26例，足月儿10例）69份脑脊液中维生素B-6的浓度。CSF样本，取自皮下脑室内水库，床边冷冻，避光。结果：吡哆醛（PL），磷酸吡哆醛（PLP），吡哆酸（PA），吡哆胺（PM）的浓度在早产儿（月经后年龄30-37周）至少是两倍高，在老年新生儿（月经后年龄>= 42周）。所有新生儿的吡哆醇和磷酸吡哆胺浓度均低于定量限。2例极早产儿（经后年龄）的CSF中
BACKGROUND AND OBJECTIVE: Vitamin B-6 plays a pivotal role in brain development and functioning. Differences in vitamin B-6 homeostasis between preterm and term newborn infants have been reported. The authors sought to investigate whether B-6 vitamers in cerebrospinal fluid (CSF) of preterm and term newborn infants are different.METHODS: B-6 vitamer concentrations were determined in 69 CSF samples of 36 newborn infants (26 born preterm and 10 born term) by ultra performance liquid chromatography-tandem mass spectrometry. CSF samples, taken from a subcutaneous intraventricular reservoir, were bedside frozen and protected from light.RESULTS: Concentrations of pyridoxal (PL), pyridoxal phosphate (PLP), pyridoxic acid (PA), and pyridoxamine (PM) in preterm newborns (postmenstrual age 30-37 weeks) were at least twice as high as in older newborns (postmenstrual age >= 42 weeks). Pyridoxine and pyridoxamine phosphate concentrations were below limits of quantification in all newborns. In CSF of 2 very preterm newborns (postmenstrual age