A selective sweep of >8 Mb on chromosome 26 in the Boxer genome
A selective sweep of >8 Mb on chromosome 26 in the Boxer genome
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DOI：
10.1186/1471-2164-12-339
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发表时间：
2011-07-01
期刊：
影响因子：
4.4
通讯作者：
Francino, Olga
中科院分区：
文献类型：
--
作者：
Quilez, Javier;Short, Andrea D.;Francino, Olga
背景资料：现代犬种显示出的性状要么是品种特异性的，要么是少数品种共有的，这是由于品种创造过程中的遗传瓶颈和品种标准的人工选择。在基因组中的选择性扫描的结果从强选择，可以检测到作为一个给定的区域的genome.Results：纯合性，指示选择性扫描的扩展区域的多态性的减少或消除，被确定在一个全基因组扫描数据集的25拳击手从英国的基因分型在类似的20,000单核苷酸多态性（SNP）。这些区域在从不同地理位置收集的拳击手的第二数据集中进一步检查，并使用更高密度的SNP阵列（类似于170，000个SNP）进行基因分型。在1号染色体上检测到以前与犬短头畸形相关的选择性扫描。一种新的选择性扫描超过8 Mb的观察26号染色体上的拳击手和较短的区域在英国和法国斗牛犬。在其他8个犬种的171个样本和7个伊比利亚狼样本中没有发现。9号染色体上的一个区域的扩展增加杂合性重叠与先前报道的拷贝数变异（CNV），这是多态性在多个狗breeds.Conclusion：超过8 Mb的26号染色体上的选择性扫描确定在拳击手基因组。这种横扫很可能是由对感兴趣的性状的强人工选择引起的，并且可能无意中导致了对该品种的不良健康影响。此外，我们提供了两个先前描述的区域的支持性证据：1号染色体上的选择性扫描与犬短头畸形和9号染色体上的CNV多态性在多个狗品种。
Background: Modern dog breeds display traits that are either breed-specific or shared by a few breeds as a result of genetic bottlenecks during the breed creation process and artificial selection for breed standards. Selective sweeps in the genome result from strong selection and can be detected as a reduction or elimination of polymorphism in a given region of the genome.Results: Extended regions of homozygosity, indicative of selective sweeps, were identified in a genome-wide scan dataset of 25 Boxers from the United Kingdom genotyped at similar to 20,000 single-nucleotide polymorphisms (SNPs). These regions were further examined in a second dataset of Boxers collected from a different geographical location and genotyped using higher density SNP arrays (similar to 170,000 SNPs). A selective sweep previously associated with canine brachycephaly was detected on chromosome 1. A novel selective sweep of over 8 Mb was observed on chromosome 26 in Boxer and for a shorter region in English and French bulldogs. It was absent in 171 samples from eight other dog breeds and 7 Iberian wolf samples. A region of extended increased heterozygosity on chromosome 9 overlapped with a previously reported copy number variant (CNV) which was polymorphic in multiple dog breeds.Conclusion: A selective sweep of more than 8 Mb on chromosome 26 was identified in the Boxer genome. This sweep is likely caused by strong artificial selection for a trait of interest and could have inadvertently led to undesired health implications for this breed. Furthermore, we provide supporting evidence for two previously described regions: a selective sweep on chromosome 1 associated with canine brachycephaly and a CNV on chromosome 9 polymorphic in multiple dog breeds.