Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues
Cellular and subcellular localization of ADP-ribosylation factor 6 in mouse peripheral tissues
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小鼠外周组织中 ADP-核糖基化因子 6 的细胞和亚细胞定位
DOI：
10.1007/s00418-017-1599-8
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发表时间：
2017
影响因子：
2.3
通讯作者：
Sakagami Hiroyuki
中科院分区：
文献类型：
--
作者：
Katsumata Osamu;Mori Momoko;Sawane Yusuke;Niimura Tomoko;Ito Akiko;Okamoto Hirotsugu;Fukaya Masahiro;Sakagami Hiroyuki
adp核糖基化因子6 （Arf6）是一种小的GTPase，调节内体运输和肌动蛋白细胞骨架重塑。本研究采用Tris-EDTA缓冲液（pH 9.0）热诱导抗原回收法，采用免疫荧光和免疫电镜综合检测了Arf6在成年小鼠外周组织中的细胞和亚细胞定位。在食管、胃、小肠和大肠、气管、肾脏、附睾、输卵管和子宫等组织的上皮细胞中观察到明显的Arf6免疫标记。在大多数简单或假分层上皮细胞中，Arf6主要定位于基底外侧膜和内体亚群。在电镜水平上，Arf6定位于基底外侧膜，在交叉突和包涵处密集聚集。Arf6在睾丸精原细胞和精母细胞的细胞间桥以及卵巢卵泡、胸腺和脾脏的细胞动力学体细胞弗莱明体中以环状形式存在。本研究为了解Arf6在全动物水平上的生理作用提供了解剖学线索。
ADP-ribosylation factor 6 (Arf6) is a small GTPase that regulates endosomal trafficking and actin cytoskeleton remodeling. In the present study, we comprehensively examined the cellular and subcellular localization of Arf6 in adult mouse peripheral tissues by immunofluorescence and immunoelectron microscopy using the heat-induced antigen retrieval method with Tris–EDTA buffer (pH 9.0). Marked immunolabeling of Arf6 was observed particularly in epithelial cells of several tissues including the esophagus, stomach, small and large intestines, trachea, kidney, epididymis, oviduct, and uterus. In most epithelial cells of simple or pseudostratified epithelia, Arf6 exhibited predominant localization to the basolateral membrane and a subpopulation of endosomes. At an electron microscopic level, Arf6 was localized along the basolateral membrane, with dense accumulation at interdigitating processes and infoldings. Arf6 was present in a ring-like appearance at intercellular bridges in spermatogonia and spermatocytes in the testis and at the Flemming body of cytokinetic somatic cells in the ovarian follicle, thymus, and spleen. The present study provides anatomical clues to help understand the physiological roles of Arf6 at the whole animal level.