Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies
Effect of multiple freeze-thaw cycles on detection of measles, mumps, and rubella virus antibodies
复制标题
DOI：
10.1128/cdli.10.1.19-21.2003
复制
发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Poland, GA
中科院分区：
文献类型：
--
作者：
Pinsky, NA;Huddleston, JM;Poland, GA
我们用全病毒免疫球蛋白G酶联免疫测定法研究了多次冻融循环对腮腺炎、麻疹和风疹病毒血清抗体水平的影响。将来自9名健康成年志愿者的新鲜血清样品分成6组，每组5份。通过总共10次冻融循环采集样品，并在4 ℃下储存直至测定。每次测定重复进行5次，并报告平均值。在完成10次冻融循环后，我们发现对测量的抗体水平没有临床或统计学显著影响，并且发现对通过酶联免疫测定法测量这些抗体的能力没有明显的有害影响。
We investigated the effect of multiple freeze-thaw cycles on mumps, measles, and rubella virus serum antibody levels with whole-virus immunoglobulin G enzyme-linked immunoassays. Fresh serum samples from nine healthy adult volunteers were divided into six sets of five aliquots each. Samples were taken through a total of 10 freeze-thaw cycles and stored at 4degreesC until assayed. Each assay measurement was done in replicates of five, and the mean value was reported. After completing 10 freeze-thaw cycles, we found no clinically or statistically significant effect on measured antibody levels and found no discernible detrimental effect on the ability to measure these antibodies by enzyme-linked immunoassays.