De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes.
De novo assembly and characterization of the Chinese three-keeled pond turtle (Mauremys reevesii) transcriptome: presence of longevity-related genes.
复制标题
中国三龙骨池龟（Mauremys reevesii）转录组的从头组装和表征：长寿相关基因的存在
作者：
Yin H;Nie L;Zhao F;Zhou H;Li H;Dong X;Zhang H;Wang Y;Shi Q;Li J
瑞氏拟水龟（Mauremys reevesii）是东亚最常见、分布最广的半水生龟类之一。一些个体的寿命异常长，这使得这种海龟物种成为研究长寿分子基础的潜在有用的模式生物。本研究采用Illumina Hiseq 2500测序平台对3名成年个体的肝脏、脾脏和肌肉组织中提取的总RNA进行了测序。在转录组中发现了一组端粒相关基因，包括tert、tep1和6个shelterin复合物蛋白编码基因（trf1、trf2、tpp1、pot1、tin2和rap1）。这些基因产物保护染色体末端免受退化，因此对海龟的寿命有很大的贡献。本研究中产生的转录组数据为海龟未来的分子研究提供了全面的参考。
Mauremys reevesii (Geoemydidae) is one of the most common and widespread semi-aquatic turtles in East Asia. The unusually long lifespan of some individuals makes this turtle species a potentially useful model organism for studying the molecular basis of longevity. In this study, pooled total RNA extracted from liver, spleen and skeletal-muscle of three adult individuals were sequenced using Illumina Hiseq 2500 platform. A set of telomere-related genes were found in the transcriptome, including tert, tep1, and six shelterin complex proteins coding genes (trf1, trf2, tpp1, pot1, tin2 and rap1). These genes products protect chromosome ends from deterioration and therefore significantly contribute to turtle longevity. The transcriptome data generated in this study provides a comprehensive reference for future molecular studies in the turtle.