INITIATION OF ROLLING-CIRCLE REPLICATION IN PT181 PLASMID - INITIATOR PROTEIN ENHANCES CRUCIFORM EXTRUSION AT THE ORIGIN
INITIATION OF ROLLING-CIRCLE REPLICATION IN PT181 PLASMID - INITIATOR PROTEIN ENHANCES CRUCIFORM EXTRUSION AT THE ORIGIN
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DOI：
10.1073/pnas.87.21.8560
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发表时间：
1990-11-01
影响因子：
11.1
通讯作者：
NOVICK, RP
中科院分区：
文献类型：
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作者：
NOIROT, P;BARGONETTI, J;NOVICK, RP
用核酸酶S1消化和体内溴乙醛处理对pT181 DNA二级结构进行了体外分析。在体外，pT181复制起始处的十字形结构通过与质粒编码的启动子蛋白RepC的结合而大大增强。在体内，复制起始处也存在DNA二级结构。其形成频率与RepC的利用效率相关。这些数据表明，起始处的十字形挤压参与了pT181滚环复制的启动。在体内，邻近的DNA结构会影响起始物的构象。
Plasmid pT181 DNA secondary structures have been analyzed in vitro by nuclease S1 digestion and in vivo by bromoacetaldehyde treatment. A cruciform structure occurring at the pT181 replication origin in vitro is greatly enhanced by the binding of the plasmid-encoded initiator protein RepC. In vivo a DNA secondary structure also existed in the replication origin. Its frequency of formation was correlated with efficiency of RepC utilization. These data suggest that cruciform extrusion at the origin is involved in initiation of pT181 rolling-circle replication. A neighboring DNA structure influences the conformation of the origin in vivo.