GLUTAMATE-LIKE IMMUNOREACTIVITY AND FATE OF CAJAL-RETZIUS CELLS IN THE MURINE CORTEX AS IDENTIFIED WITH CALRETININ ANTIBODY
GLUTAMATE-LIKE IMMUNOREACTIVITY AND FATE OF CAJAL-RETZIUS CELLS IN THE MURINE CORTEX AS IDENTIFIED WITH CALRETININ ANTIBODY
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DOI：
10.1093/cercor/5.1.13
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发表时间：
1995-01-01
期刊：
影响因子：
3.7
通讯作者：
SORIANO, E
中科院分区：
文献类型：
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作者：
DELRIO, JA;MARTINEZ, A;SORIANO, E
皮质层 VI 至 II 在两层较老的神经元（边缘区和亚板区）之间发育，人们认为它们在皮质发育中具有独特的作用。虽然已发现亚板细胞对于丘脑皮质关系的建立至关重要，但边缘区的功能，特别是 Cajal-Retzins 神经元的功能尚未阐明。在这里，我们展示了针对钙结合蛋白钙结合蛋白的抗体在小鼠大脑皮层中的整个生命周期中标记了 Cajal-Retzius 细胞群。在产前和产后早期，发现 Cajal-Retzius 细胞均匀分布在整个小鼠大脑皮层中。在发育中的海马和齿状回中也发现了类似 Cajal-Retzius 的神经元，这表明它们可能在皮质发育中具有一般功能。从 P8 开始，Cajal-Retzius 细胞从新皮质和海马消失，同时观察到退化的免疫反应性神经元。钙结合蛋白阳性的 Cajal-Retzius 细胞具有谷氨酸免疫反应性，并且其假定的轴突末端形成不对称突触。这些观察结果表明，Cajal-Retzius 细胞可能提供强直兴奋性输入，这对于皮质神经元的成熟至关重要。此外，由于神经元迁移已被证明依赖于谷氨酸受体，我们提出释放谷氨酸的 Cajal-Retzius 细胞可能会引导神经母细胞向边缘层迁移，从而产生皮质发育的“从内到外”序列，
Cortical layers VI to II develop between two layers of older neurons, the marginal and subplate zones, which are believed to have unique roles in cortical development, While subplate cells have been found essential for the establishment of thalamocortical relationships, the function of the marginal zone and in particular of the neurons of Cajal-Retzins has not been elucidated. Here we show that an antibody against the calcium-binding protein calretinin labels the population of Cajal-Retzius cells throughout their life in the murine cerebral cortex. In prenatal and early postnatal stages, Cajal-Retzius cells were found evenly distributed throughout the murine cerebral cortex. Cajal-Retzius-like neurons were also found in the developing hippocampus and dentate gyrus, which indicates that they may have a general function in cortical development. From P8 onward Cajal-Retzius cells disappeared from the neocortex and hippocampus, at the same time as degenerating immunoreactive neurons were observed. Calretinin-positive Cajal-Retzius cells were glutamate immunoreactive and their presumed axon terminals formed asymmetric synapses. These observations indicate that Cajal-Retzius cells may provide a tonic excitatory input, essential for the maturation of cortical neurons. Furthermore, since neuronal migration has been shown to be dependent on glutamate receptors, we propose that Cajal-Retzius cells releasing glutamate may direct migrating neuroblasts toward the marginal lamina, therefore creating the ''inside-out'' sequence of cortical development,