Enhanced bone regeneration by silicon-substituted hydroxyapatite derived from cuttlefish bone
Enhanced bone regeneration by silicon-substituted hydroxyapatite derived from cuttlefish bone
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DOI：
10.1111/clr.12613
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发表时间：
2017-01-01
影响因子：
4.3
通讯作者：
Lee, Jun
中科院分区：
文献类型：
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作者：
Kim, Beom-Su;Yang, Sun-Sik;Lee, Jun
目的乌贼骨(CB)作为一种骨移植材料越来越受到人们的关注。硅在骨形成和钙化过程中起着重要作用。本研究旨在利用天然炭黑制备硅取代炭黑衍生羟基磷灰石(Si-CB-HAP)，以提高其成骨生物活性。材料和方法以炭黑为原料，采用水热和溶剂热法制备了Si-CB-HAP。用扫描电子显微镜(SEM)、X射线衍射仪(X射线衍射仪)和能谱仪(EDS)对涂层的微观结构和化学成分进行了表征。用人骨髓间充质干细胞评价了Si-CB-HAP的生物活性。结果表明，Si-CB-HAP中的硅含量为0.77wt%，保持了原有的显微结构。硅的存在可以促进人骨髓间充质干细胞的增殖和早期细胞贴壁。此外，碱性磷酸酶活性和成骨细胞标志基因的实时定量聚合酶链式反应结果表明，CB-HAP中的硅替代促进了成骨细胞的分化。另外，在体骨缺损修复实验中，Si-CB-HAP的成骨能力明显高于CB-HAP。结论Si-CB-HAP有望作为骨移植材料用于促进骨愈合。
ObjectiveThere is growing interest in the use of cuttlefish bone (CB) as a bone graft material. Silicon (Si) plays an important role in bone formation and calcification. This study aimed to prepare Si-substituted CB-derived hydroxyapatite (Si-CB-HAp) using a natural CB to improve the bioactivity for bone formation.Materials and methodsWe prepared Si-HAp from CB (Si-CB-HAp) using a hydrothermal and solvothermal method. The microstructure and chemical composition were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and energy dispersive X-ray spectrometer (EDS). The bioactivity of the Si-CB-HAp was evaluated using human mesenchymal stem cells. Furthermore, the invivo bone regeneration efficiency was evaluated using a rabbit calvarial defect model.ResultsOur results show that the Si content was 0.77 wt% in Si-CB-HAp, and its original microstructure was conserved. The presence of Si was shown to enhance cell proliferation and early cellular attachment of human mesenchymal stem cells. Additionally, results of alkaline phosphatase activity and real-time PCR for osteoblast marker genes show that Si substitution into CB-HAp enhanced osteoblast differentiation. In addition, invivo bone defect healing experiments show that the formation of bone with Si-CB-HAp is higher than that with CB-HAp.ConclusionThese results indicate that Si-CB-HAp may potentially be used as a bone graft material to enhance bone healing.