Electron flow through proteins
Electron flow through proteins
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DOI：
10.1016/j.cplett.2009.10.051
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发表时间：
2009-11-24
影响因子：
2.8
通讯作者：
Winkler, Jay R.
中科院分区：
文献类型：
--
作者：
Gray, Harry B.;Winkler, Jay R.
光合作用和呼吸作用中的电子转移通常发生在相隔较大分子距离的含金属辅因子之间。利用激光闪灭触发方法，我们已经证明，在溶液和晶体中，ru修饰的细胞色素和蓝铜蛋白中，20埃、耦合受限的Fe-II-Ru-III和Cu-I-Ru-III电子隧穿可以在微秒时间尺度上发生。氧化还原等价物可以通过多步隧穿（通常称为跳跃）通过介入的氨基酸侧链转移到更远的距离。我们的工作已经确定，通过中间色氨酸的20埃空穴跳变比在重新修饰的蓝铜蛋白中单步电子隧穿快两个数量级。(C) 2009 Elsevier B.V.版权所有
Electron transfers in photosynthesis and respiration commonly occur between metal-containing cofactors that are separated by large molecular distances. Employing laser flash-quench triggering methods, we have shown that 20-angstrom, coupling-limited Fe-II-Ru-III and Cu-I-Ru-III electron tunneling in Ru-modified cytochromes and blue copper proteins can occur on the microsecond timescale both in solutions and crystals. Redox equivalents can be transferred even longer distances by multistep tunneling, often called hopping, through intervening amino acid side chains. Our work has established that 20-angstrom hole hopping through an intervening tryptophan is two orders of magnitude faster than single-step electron tunneling in a Re-modified blue copper protein. (C) 2009 Elsevier B.V. All rights reserved.