Hepatic lineages isolated from developing rat liver show different ways of maturation
Hepatic lineages isolated from developing rat liver show different ways of maturation
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DOI：
10.1016/s0006-291x(03)00662-4
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发表时间：
2003-05-23
影响因子：
3.1
通讯作者：
Kluth, D
中科院分区：
文献类型：
--
作者：
Fiegel, HC;Kluth, J;Kluth, D
免疫细胞化学分析显示，在肝脏发育过程中存在不同的肝细胞类型：（i）共表达干细胞标志物Thy I和肝谱系标志物CK-18的细胞和（ii）仅表达CK-18的细胞（肝母细胞）。在这项研究中，我们分离了不同的肝细胞，并分析了基因表达和表型。用胶原酶消化胎鼠肝脏。OX 43-和OX 44-阳性造血细胞被耗尽，并且使用磁性细胞分选富集Thy 1-阳性细胞。通过RT-PCR和免疫细胞化学分析不同细胞室的Thy 1、CK-18、AFP和白蛋白。成肝细胞表达白蛋白在所有时间和AFP在早期阶段。Thy 1富集细胞在所有时间表达CK-18，早期表达白蛋白，晚期表达AFP。来自胎肝的Thy 1阳性细胞表达肝脏特异性基因。这些数据表明，Thy I阳性肝细胞向肝干细胞发育，成肝细胞向成人肝的成熟肝细胞发育。(C)2003 Elsevier Science（美国）。All rights reserved.
Immunocytochemical analysis revealed that different hepatic cell types exist during liver development: (i) cells co-expressing the stem-cell marker Thy I and the hepatic lineage marker CK-18 and (ii) cells only expressing CK-18 (hepatoblasts). In this study we separated the different hepatic cells and analyzed gene-expression and phenotype. Fetal rat livers were digested by collagenase solution. OX43- and OX44-positive hematopoietic cells were depleted and Thy1-positive cells were enriched using Magnetic cell sorting. The different cell compartments were analyzed by RT-PCR and immunocytochemistry for Thy 1, CK-18, AFP, and albumin. Hepatoblasts expressed albumin at all times and AFP in the early stages. Thy1-enriched cells expressed CK-18 at all times, albumin in the early, and AFP in the late stages. Thy1-positive cells from fetal livers express liver specific genes. The data suggest that Thy I positive hepatic cells develop towards hepatic stem cells, and hepatoblasts develop towards mature hepatocytes of the adult liver. (C) 2003 Elsevier Science (USA). All rights reserved.