Genetic variability in Macadamia
Genetic variability in Macadamia
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DOI：
10.1023/a:1008634103954
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发表时间：
1998-02-01
影响因子：
2
通讯作者：
Manshardt, RM
中科院分区：
文献类型：
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作者：
Aradhya, MK;Yee, LK;Manshardt, RM
对澳洲坚果4个种45份材料的遗传变异进行了分析。integrifolia，M. tetraphylla、M. ternifolia和M. hildebrandii和野生近缘种，Hicksbeachia pinnatifolia使用由16个位点编码的8个酶系统（Gpi-1和2，Idh-1和2，Lap，Mdh-1，2和3，6Pgd-2，Pgm-2和3，Tpi-1和2，Ugpp-1，2和3）进行。43份材料具有明显的同工酶指纹图谱，表明其遗传变异程度较高。利用聚类分析的多变量关系的加入检查导致四个集群的识别，其中两个包含一个加入每个代表H。pinnatifolia和M.希尔德布兰德。所有的夏威夷栽培品种被包括在最大的集群内的两个亚集群中，该集群包括所有的M。integrifolia和M.这表明：（1）夏威夷栽培品种必须起源于至少两个遗传多样性的祖先群体和（2）M。ternifolia可能是M.全缘叶Macadamia tetraphylla与M. integrifolia和M. ternifolia复合体表明这些类群代表一个种复合体。遗传变异的不同措施，如平均每个位点的等位基因数，多态性指数，观察到的和预期的杂合性水平，表明在澳洲坚果收集的遗传变异的显着水平。
A genetic variability analysis involving 45 accessions of Macadamia including four species, M. integrifolia, M. tetraphylla, M. ternifolia, and M. hildebrandii and a wild relative, Hicksbeachia pinnatifolia was performed using eight enzyme systems encoded by 16 loci (Gpi-1 and 2, Idh-1 and 2, Lap, Mdh-1, 2, and 3, 6Pgd-2, Pgm-2 and 3, Tpi-1 and 2, Ugpp-1, 2, and 3). Forty-three accessions possessed distinct isozyme fingerprints indicating a high level of genetic variation. Examination of multivariate relationships among accessions using a cluster analysis resulted in the identification of four clusters, two of which contained one accession each representing H. pinnatifolia and M. hildebrandii. All Hawaiian cultivars were included in two sub-clusters within the largest cluster, which encompassed all the M. integrifolia and three M. ternifolia accessions, suggesting that: (I) the Hawaiian cultivars must have originated from at least two genetically diverse ancestral populations and (2) M. ternifolia may be a conspecific variant of M. integrifolia. Macadamia tetraphylla has diverged marginally from the M. integrifolia and M. ternifolia complex suggesting that these taxa represent a species complex. The different measures of genetic variability such as mean number of alleles per locus, polymorphic index, and observed and expected levels of heterozygosity, indicated significant levels of genetic variation in the Macadamia collection.