Cloning and functional expression of the human type 1 sigma receptor (hSigmaR1)
Cloning and functional expression of the human type 1 sigma receptor (hSigmaR1)
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DOI：
10.1006/bbrc.1996.1842
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发表时间：
1996-12-13
影响因子：
3.1
通讯作者：
Ganapathy, V
中科院分区：
文献类型：
--
作者：
Kekuda, R;Prasad, PD;Ganapathy, V
我们从人胎盘绒毛膜癌细胞（JAR） cDNA文库中分离出一个编码功能性1型sigma受体的cDNA克隆。利用豚鼠sigma受体cDNA特异性引物从豚鼠肾脏mRNA中获得RT-PCR产物，筛选cDNA文库。hSigmaR1 cDNA预测了223个氨基酸的蛋白质，具有单一的跨膜结构域。该氨基酸序列与豚鼠sigma受体有93%的同源性。当在HeLa细胞中功能性表达时，hSigmaR1 cDNA增强了sigma受体配体[H-3]-氟哌啶醇与HeLa细胞膜的结合。克隆的hSigmaR1的抑制剂特异性表明它是1型sigma受体。包括胎盘、肝脏和大脑在内的多种人体组织以及几种人类细胞系不同程度地表达SigmaR1 mRNA （1.7 kb）。(C) 1996学术出版社，Inc.
We have isolated a cDNA clone from a human placental choriocarcinoma cell (JAR) cDNA library which codes for a functional type 1 sigma receptor. An RT-PCR product obtained from guinea pig kidney mRNA using primers specific for the guinea pig sigma receptor cDNA was used to screen the cDNA library. The hSigmaR1 cDNA predicts a protein of 223 amino acids with a single putative transmembrane domain. The amino acid sequence exhibits 93% identity with the guinea pig sigma receptor. When functionally expressed in HeLa cells, the hSigmaR1 cDNA enhances the binding of [H-3]-haloperidol, a sigma receptor ligand, to the HeLa cell membranes. The inhibitor specificity of the cloned hSigmaR1 indicates that it is the type 1 sigma receptor. Several human tissues, including placenta, liver, and brain, and several human cell lines express the SigmaR1 mRNA (1.7 kb) to a variable extent. (C) 1996 Academic Press, Inc.