Conformational and Phase Transitions in DNA-Photosensitive Surfactant Solutions: Experiment and Modeling
Conformational and Phase Transitions in DNA-Photosensitive Surfactant Solutions: Experiment and Modeling
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DOI：
10.1002/bip.22575
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发表时间：
2015-02-01
期刊：
影响因子：
2.9
通讯作者：
Unksov, I.
中科院分区：
文献类型：
--
作者：
Kasyanenko, N.;Lysyakova, L.;Unksov, I.
采用动态光散射法（DLS）、低梯度粘度法（LGV）、原子力显微镜（AFM）、圆二色法（CD）、凝胶电泳法（GE）、流动双折射法（FB）、紫外-可见分光光度法（UV-Vis）研究了DNA与含偶氮苯阳离子表面活性剂反式和顺式异构体在5 mM NaCl溶液中的结合。研究了DNA与光敏表面活性剂配合物的光响应构象转变、DNA光学各向异性和持续长度的变化、高浓度表面活性剂诱导DNA向纳米颗粒的相变以及表面活性剂与大分子结合时的构象转变。用计算机模拟了含表面活性剂偶氮苯的顺式和反式异构体的胶束形成以及dna -表面活性剂的相互作用。设计了dna -表面活性剂溶液的相图。用稀释剂和光照射可以逆转表面活性剂结合诱导的DNA包装。(c) 2014 Wiley期刊公司生物工程学报（自然科学版），2015。
DNA binding to trans- and cis-isomers of azobenzene containing cationic surfactant in 5 mM NaCl solution was investigated by the methods of dynamic light scattering (DLS), low-gradient viscometry (LGV), atomic force microscopy (AFM), circular dichroism (CD), gel electrophoresis (GE), flow birefringence (FB), UV-Vis spectrophotometry. Light-responsive conformational transitions of DNA in complex with photosensitive surfactant, changes in DNA optical anisotropy and persistent length, phase transition of DNA into nanoparticles induced by high surfactant concentration, as well as transformation of surfactant conformation under its binding to macromolecule were studied. Computer simulations of micelles formation for cis- and trans-isomers of azobenzene containing surfactant, as well as DNA-surfactant interaction, were carried out. Phase diagram for DNA-surfactant solutions was designed. The possibility to reverse the DNA packaging induced by surfactant binding with the dilution and light irradiation was shown. (c) 2014 Wiley Periodicals, Inc. Biopolymers 103: 109-122, 2015.