CD34+CDw90(Thy-1)+ subset colocated with mesenchymal progenitors in human normal bone marrow hematon units is enriched in colony-forming unit megakaryocytes and long-term culture-initiating cells
CD34+CDw90(Thy-1)+ subset colocated with mesenchymal progenitors in human normal bone marrow hematon units is enriched in colony-forming unit megakaryocytes and long-term culture-initiating cells
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DOI：
10.1016/j.exphem.2003.08.010
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发表时间：
2003-12-01
影响因子：
2.6
通讯作者：
Berger, MG
中科院分区：
文献类型：
--
作者：
Boiret, N;Rapatel, C;Berger, MG
客观的。同种异体移植的支持治疗和成人骨髓 (BM) 中间充质干细胞的鉴定所取得的进展，引起了人们对使用 BM 作为细胞治疗形式的新兴趣。为了优化 BM 细胞的收集，我们评估了 BM 血红单位 (HU) 的造血和间充质未成熟细胞含量，这些细胞通常在移植过程中被消除。材料和方法。在短期培养中对来自 HU 和血沉棕黄层 (BC) 区室的造血 CD34(+) 祖细胞进行了表征。在集落形成细胞 (CFC) 和长期培养起始细胞 (LTC-IC) 测定中评估分选的 CD34(+)CDw90(Thy-1)(+) 原始子集，然后通过其他抗原的表达进一步表征。同时，我们评估了集落形成单位成纤维细胞 (CFU-F) 数量并对新鲜贴壁 (D1-3) 细胞进行表型分析。结果。 HU 和 BC 来源的 CD34(+) 细胞的铺板效率相同。然而，HU CD34(+)CDw90(Thy-1)(+) 子集在集落形成单位巨核细胞 (2.3x)、ITC-IC (4.6x) 和共表达 CD105 的细胞 (5x) 中富集。我们发现 CFU-F (4.7x) 的频率较高，被认为是含有间充质干细胞的群体，与新鲜贴壁 (CD45/GPA)(-)CD14(-) 细胞的富集相关。结论。我们首次表明CD34(+)CDw90(+)子集的功能特性与其在HU中的体内位置有关，HU可能代表BM间充质储备室。 HU 中 CD34(+) 细胞、CD34(+)CDw90(+) LTC-IC 和 CFU-F 的位置分别为 35.6%、59.1% 和 58.7%，证明了开发收集它们以减少治疗性 BM 体积的程序的合理性。 (C) 2003 年国际实验血液学学会。由爱思唯尔公司出版
Objective. The progress made in the supportive care of allografts and the identification of mesenchymal stem cells in adult human bone marrow (BM) has prompted renewed interest in the use of BM as a form of cell therapy. With the aim of optimizing the collection of BM cells, we evaluated the hematopoietic and mesenchymal immature cell contents of BM hematon units (HUs), which usually are eliminated during graft processing.Materials and Methods. Hematopoietic CD34(+) progenitors from HU and buffy coat (BC) compartments were characterized in short-term culture. The sorted CD34(+)CDw90(Thy-1)(+) primitive subset was assessed in colony-forming cell (CFC) and long-term culture-initiating cell (LTC-IC) assays, then further characterized by the expression of additional antigens. In parallel, we evaluated the colony-forming unit fibroblast (CFU-F) number and phenotyped the fresh adherent (D1-3) cells.Results. The plating efficiencies of CD34(+) cells derived from HU and BC were identical. However, the HU CD34(+)CDw90(Thy-1)(+) subset was enriched in colony-forming unit megakaryocyte (2.3x), ITC-IC (4.6x), and cells coexpressing CD105 (5x). We found a higher frequency of CFU-F (4.7x), considered to be the mesenchymal stem cell-containing population, correlated with an enrichment in fresh adherent (CD45/GPA)(-)CD14(-) cells.Conclusions. We show for the first time that functional properties of the CD34(+)CDw90(+) subset are related to its in vivo location in HU, which may represent the BM mesenchymal reserve compartment. The location in HU of 35.6%,59.1 %, and 58.7% of CD34(+) cells, CD34(+)CDw90(+) LTC-IC, and CFU-F, respectively, justifies the development of a procedure to collect them in order to reduce the therapeutic BM volume. (C) 2003 International Society for Experimental Hematology. Published by Elsevier Inc.