Lipase Immobilization on Hyper-Cross-Linked Polymer-Coated Silica for Biocatalytic Synthesis of Phytosterol Esters with Controllable Fatty Acid Composition
Lipase Immobilization on Hyper-Cross-Linked Polymer-Coated Silica for Biocatalytic Synthesis of Phytosterol Esters with Controllable Fatty Acid Composition
复制标题
超交联聚合物包被二氧化硅上的脂肪酶固定化生物催化合成脂肪酸组成可控的植物甾醇酯
DOI：
10.1021/jf3042962
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发表时间：
2013-01-09
影响因子：
6.1
通讯作者：
Deng, Qian-Chun
中科院分区：
文献类型：
--
作者：
Zheng, Ming-Ming;Lu, Yong;Deng, Qian-Chun
本研究以SiO2颗粒为原料，通过悬浮聚合法制备SiO2@P（MAA-co-VBC-co-DVB）前驱体，并通过超交联反应制备了SiO2@P（MAA-co-VBC-co-DVB）复合材料。以SiO2@P（MAA-co-VBC-co-DVB）为载体，通过疏水和弱阳离子交换作用固定化皱褶假丝酵母脂肪酶（CRL）。所得固定化CRL显示出更好的热稳定性和可重复使用性相比，游离CRL。在此基础上，建立了植物甾醇与不同脂肪酸高效酶催化酯化制备相应植物甾醇酯的方法。在优化的工艺条件下，以30 mL异辛烷为溶剂，80 μ mol/mL植物甾醇、160 μ mol/mL亚麻酸和15 mg/mL CRL@HPCS为原料，在300 rpm、50 ℃下反应7 h，得到6种转化率大于92.1%、脂肪酸组成可控的植物甾醇酯。所制备的植物甾醇酯具有较低的酸值（
In this study, a novel mixed-mode composite material, SiO2@P(MAA-co-VBC-co-DVB), was prepared via the hyper-cross-linking of its precursor, which was produced via suspension polymerization in the presence of SiO2 particles. Candida rugosa lipase (CRL) was immobilized on the SiO2@P(MAA-co-VBC-co-DVB) particles via hydrophobic and weak cation-exchange interaction. The resulting immobilized CRL showed much better thermal stability and reusability in comparison to free CRL. On the basis of the excellent biocatalyst prepared, a method for high-efficiency enzymatic esterification of phytosterols with different fatty acids to produce the corresponding phytosterol esters was developed. Six phytosterol esters with conversions above 92.1% and controllable fatty acid composition were obtained under the optimized conditions: 80 mu mol/mL phytosterols, 160 mu mol/mL linolenic acid, and 15 mg/mL CRL@HPCS at 300 rpm and 50 degrees C for 7 h in 30 mL of isooctane. The prepared phytosterol esters possessed a low acid value (