Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced increase in vascular permeability in mouse skin by lipoxygenase inhibitors.
Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced increase in vascular permeability in mouse skin by lipoxygenase inhibitors.
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脂氧合酶抑制剂抑制 12-O-十四烷酰佛波醇-13-乙酸酯诱导的小鼠皮肤血管通透性增加。
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
R. Kato
中科院分区：
文献类型：
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作者：
T. Nakadate;Satoshi Yamamoto;E. Aizu;R. Kato
用12- o -十四烷醇-13-醋酸酯（TPA） （0.2-2.5 nmol/只）涂敷小鼠背侧皮肤后，血管通透性呈剂量相关增加，通过TPA处理后5小时皮肤中有波胺天蓝色渗出来测定。在TPA治疗5小时后，皮肤间质中也观察到明显的中性粒细胞浸润。去甲双氢愈创木酸（NDGA）治疗小鼠（10 μ mol/小鼠）。2,3,5-三甲基-6-（12-羟基-5,10-十二烷基）-1,4-苯醌（AA861） （10 μ mol/小鼠）和槲皮素（3 μ mol/小鼠）显著抑制tpa诱导的染料渗出。而吲哚美辛（250 ~ 1000 nmol/只）对tpa诱导的染料渗出有抑制作用，但抑制作用无统计学意义。用AA861 （10 μ mol/小鼠）处理也能显著抑制tpa诱导的中性粒细胞浸润。槲皮素、NDGA和AA861对表皮脂氧合酶活性有抑制作用，而吲哚美辛对表皮脂氧合酶活性无抑制作用。另一方面，吲哚美辛对表皮环氧合酶有抑制作用，槲皮素、NDGA和AA861对表皮环氧合酶无抑制作用。本研究提示脂氧合酶产物参与了tpa诱导小鼠背侧皮肤血管通透性增加的机制。
The painting of mouse dorsal skin with 12-O-tetradecanoylphorbol-13-acetate (TPA) (0.2-2.5 nmol/mouse) induced a dose-related increase in vascular permeability, which was determined by pontamine sky blue exudation into the skin 5 hr after the TPA treatment. Marked infiltration of neutrophils in the dermal interstitium was also observed 5 hr after TPA treatment. Treatment of mice with nordihydroguaiaretic acid (NDGA) (10 mumol/mouse). 2,3,5-trimethyl-6-(12-hydroxy-5,10-dodecadiynyl)-1,4-benzoquinone (AA861) (10 mumol/mouse) and quercetin (3 mumol/mouse) significantly inhibited the TPA-induced dye exudation. However, indomethacin (250-1000 nmol/mouse) tended to inhibit the TPA-induced dye exudation, but the inhibition was not statistically significant. Treatment with AA861 (10 mumol/mouse) also caused a marked inhibition of TPA-induced neutrophil infiltration. Quercetin, NDGA and AA861 inhibited epidermal lipoxygenase activity, but indomethacin failed to inhibit it. On the other hand, indomethacin inhibited epidermal cyclooxygenase, but quercetin, NDGA and AA861 failed to inhibit it. The present study suggests involvement of a lipoxygenase product(s) in the mechanism of the TPA-induced increase in vascular permeability in the dorsal skin of mice.
豚鼠皮肤中花生四烯酸的代谢。
DOI：
10.1016/0005-2760(82)90052-2
发表时间：
1982
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Ruzicka,T;Printz,MP
通讯作者：
Printz,MP