Mutant APH(2'')-IIa enzymes with increased activity against amikacin and isepamicin.
Mutant APH(2'')-IIa enzymes with increased activity against amikacin and isepamicin.
复制标题
突变型 APH(2)-IIa 酶对阿米卡星和异帕米星的活性增强。
DOI：
10.1128/aac.01444-09
复制
发表时间：
2010
影响因子：
4.9
通讯作者：
Vakulenko,SergeiB
中科院分区：
文献类型：
--
作者：
Toth,Marta;Frase,Hilary;Chow,JosephW;Smith,Clyde;Vakulenko,SergeiB
Directed evolution by random PCR mutagenesis of the gene for the aminoglycoside 2″-IIa phosphotransferase generated R92H/D268N and N196D/D268N mutant enzymes, resulting in elevated levels of resistance to amikacin and isepamicin but not to other aminoglycoside antibiotics. Increases in the activities of the mutant phosphotransferases for isepamicin are the result of decreases inKmvalues, while improved catalytic efficiency for amikacin is the result of both a decrease inKmvalues and an increase in turnover of the antibiotic. Enzymes with R92H, D268N, and D268N single amino acid substitutions did not result in elevated MICs for aminoglycosides.
