Quantitative trait loci affecting growth in high growth (hg) mice
Quantitative trait loci affecting growth in high growth (hg) mice
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DOI：
10.1007/s003350010275
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发表时间：
2001-04-01
期刊：
影响因子：
2.5
通讯作者：
Medrano, JF
中科院分区：
文献类型：
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作者：
Corva, PM;Horvat, S;Medrano, JF
进行全基因组扫描以鉴定与分离高生长（hg）的群体中的生长相关的数量性状基因座（QTL），所述高生长（hg）是一种部分隐性突变，其增强小鼠的生长速率和体型。从C57 BL/6 J-hg/hg X CAST/EiJ F-2杂交中选择262 hg/hg小鼠的样品，并用分布在基因组中的79个SSLP标记进行分型。通过区间作图，确定了8个显著位点。染色体2和8上的基因座影响F-2小鼠的生长速率。Chr 2和11上的位点影响生长速率和胴体瘦肉质量（蛋白质和灰分）。在第9代和第17代上分别有一个位点对胴体瘦肉重和股骨长有影响，但对生长速率无显著影响。Chrs 5和9上的位点改变了胴体脂肪含量。C57 BL/6 J等位基因的加性效应均为正，但影响胴体肥度的两个位点除外。在274 +/+ F-2小鼠中选择的标记的分型揭示了hg和其他生长QTL之间的显著相互作用，其被检测为基因作用（加性或显性）和等位基因置换效应的变化。有关基因座之间相互作用的知识，特别是当涉及主基因时，将有助于识别位置候选基因，并有助于理解哺乳动物生长速度和体型的复杂遗传调控。
A genome-wide scan was performed in order to identify Quantitative Trait Loci (QTL) associated with growth in a population segregating high growth (hg), a partially recessive mutation that enhances growth rate and body size in the mouse. A sample of 262 hg/hg mice was selected from a C57BL/6J-hg/hg X CAST/EiJ F-2 cross and typed with 79 SSLP markers distributed across the genome. Eight significant loci were identified through interval mapping. Loci on Chromosomes (Chrs) 2 and 8 affected the growth rate of F-2 mice. Loci on Chr 2 and 11 affected growth rate and carcass lean mass (protein and ash). A locus on Chr 9 modified femur length and another one in Chr 17 affected both carcass lean mass and femur length, but none of these had significant effects on growth rate. Loci on Chrs 5 and 9 modified carcass fat content. Additive effects were positive for C57BL/6J alleles, except for the two loci affecting carcass fatness. Typing of selected markers in 274 +/+ F-2 mice revealed significant interactions between hg and other growth QTL, which were detected as changes in gene action (additive or dominant) and in allele substitution effects. Knowledge about interactions between loci, especially when major genes are involved, will help in the identification of positional candidate genes and in the understanding of the complex genetic regulation of growth rate and body size in mammals.