Studies on pathogenic Vibrio parahaemolyticus during a warm weather season in the Seto Inland Sea, Japan
Studies on pathogenic Vibrio parahaemolyticus during a warm weather season in the Seto Inland Sea, Japan
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DOI：
10.1046/j.1462-2920.2003.00458.x
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发表时间：
2003-08-01
影响因子：
5.1
通讯作者：
Shinoda, S
中科院分区：
文献类型：
--
作者：
Alam, MJ;Miyoshi, SI;Shinoda, S
副溶血性弧菌是一种潜在的致病性细菌，在世界各地的河口和海洋环境中自然存在。这种生物在水生环境中的发病率取决于许多生态因素。在温暖的天气季节，从日本濑户内海海岸收集海水和有机材料，并对其进行分析，以确定副溶血性弧菌的密度和病原菌株的发生率，病原菌株定义为通过聚合酶链反应（PCR）从样品富集培养中分离的DNA，具有tdh和/或trh基因。采用最可能数(MPN)-PCR技术，当每100 ml水或10 g有机物样品的密度为3 ~ bb1 400个细胞时，约99%的样品呈阳性，而采用常规MPN培养技术，当每100 ml水或10 g有机物样品的密度为3 ~ bb1 400个细胞时，只有76.6%的样品呈阳性。此外，通过MPN-PCR技术，tdh和trh基因分别在41.5%和8.5%的样品中呈阳性。常规MPN培养未分离到tdh和trh基因阳性菌株。MPN培养和MPN- pcr技术之间的检测差异似乎是显著的，这可能归因于不同的检测灵敏度和其他因素。
Vibrio parahaemolyticus is a potentially pathogenic bacterium, occurring naturally in estuarine and marine environments throughout the world. The incidence of this organism in an aquatic environment depends upon many ecofactors. Sea water and organic material were collected during the warm weather season from a coast of the Seto Inland Sea, Japan, and analysed to determine V. parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by polymerase chain reaction (PCR), using isolated DNA from enrichment culture of the samples. About 99% of samples were positive for V. parahaemolyticus with densities of 3 to> 1400 cells per 100 ml of water or 10 g of organic samples by the most-probable-number (MPN)-PCR technique, but only 76.6% were positive by the conventional MPN culture technique, with densities ranging from 3 to> 1400 cells per 100 ml of water or 10 g of organics. Furthermore, the tdh and trh genes were positive in 41.5% and 8.5% of samples, respectively, by the MPN-PCR technique. No tdh and trh gene-positive strains were isolated by the conventional MPN culture procedure. The difference in detection between the MPN culture and the MPN-PCR techniques appeared to be significant and may be attributed to different detection sensitivities and other factors.