Nucleosides and nucleotides. 218. Alternate-strand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides using a purine motif.
Nucleosides and nucleotides. 218. Alternate-strand triple-helix formation by the 3'-3'-linked oligodeoxynucleotides using a purine motif.
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核苷和核苷酸。
DOI：
10.1021/bc0256493
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发表时间：
2003
影响因子：
4.7
通讯作者：
A. Matsuda
中科院分区：
文献类型：
--
作者：
S. Hoshika;Y. Ueno;A. Matsuda
在本文中，我们描述了 3'-3' 连接的 TFO 的合成，该 TFO 可以通过反向 Hoogsteen 氢键与双链体 DNA 靶标形成反平行三链体。使用电泳迁移率变动分析 (EMSA) 研究了这些 TFO 和靶 DNA 之间的交替链三链体的稳定性。结果发现，通过将 TFO 片段与季戊四醇接头连接，交替链三链体显着稳定。并且，与由嘧啶基序组成的交替链三链体不同，氨基丁基连接体的末端铵离子和TFO的嵌入剂对由嘌呤基序组成的交替链三链体的稳定性没有贡献。我们还测试了 3'-3'-连接的 TFO 抑制限制性酶 EcoT14I 对双链 DNA 靶标 17 切割的能力，并发现 3'-3'-连接的 TFO 12 和 13 比未连接的十聚体 8 更有效地抑制酶切割。因此，与季戊四醇连接的 TFO 可用作 DNA 靶标的反基因寡核苷酸，其具有交替性寡嘧啶-寡嘌呤序列。
In this paper, we describe the synthesis of the 3'-3'-linked TFOs that can form the antiparallel triplexes with the duplex DNA target by reverse Hoogsteen hydrogen bonds. Stability of the alternate-strand triplexes between these TFOs and the target DNAs was investigated using the electrophoretic mobility shift assay (EMSA). It was found that the alternate-strand triplexes were significantly stabilized by linking the TFO fragments with the pentaerythritol linker. And, unlike the alternate-strand triplexes composed of the pyrimidine motif, the terminal ammonium ion of the aminobutyl-linker and the intercalator of the TFOs did not contribute to the stability of the alternate-strand triplex comprised of the purine motif. We also tested the ability of the 3'-3'-linked TFOs to inhibit cleavage of the duplex DNA target 17 by the restriction enzyme EcoT14I and found that the 3'-3'-linked TFOs 12 and 13 inhibited the cleavage by the enzyme more effectively than the unlinked decamer 8. Thus, the TFOs linked with pentaerythritol may be useful as the antigene oligonucleotide to the DNA targets, which have alternating oligopyrimidine-oligopurine sequences.