Neuroepithelial transforming gene 1 functions as a potential prognostic marker for patients with non-small cell lung cancer
Neuroepithelial transforming gene 1 functions as a potential prognostic marker for patients with non-small cell lung cancer
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神经上皮转化基因1作为非小细胞肺癌患者的潜在预后标志物
DOI：
10.3892/mmr.2015.4385
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发表时间：
2015-11-01
影响因子：
3.4
通讯作者：
Jin, Longyu
中科院分区：
文献类型：
--
作者：
Fang, Li;Zhu, Jialiang;Jin, Longyu
非小细胞肺癌（NSCLC）是世界范围内最常见的组织学癌症亚型。神经上皮转化基因1（Net-1），Ras同源家族成员A特异性鸟嘌呤核苷酸交换因子，已被证明在几种人类癌症类型中上调。然而，Net-1在NSCLC中表达的临床意义仍然难以捉摸。本研究通过逆转录定量聚合酶链反应和蛋白质印迹分析64例非小细胞肺癌及其癌旁正常组织中Net-1的mRNA和蛋白水平。此外，通过免疫组织化学染色检测来自临床注释的I-III期NSCLC病例的肿瘤组织中的Net-1蛋白表达。结果显示，NSCLC组织中Net-1 mRNA和蛋白水平均显著高于相应的非肿瘤组织。Net-1表达与临床分期、淋巴结转移、远处转移及分化程度密切相关（P
Non-small cell lung cancer (NSCLC) is the most common histological cancer sub-type worldwide. Neuroepithelial transforming gene 1 (Net-1), a Ras homolog family member A-specific guanine nucleotide exchange factor, has been shown to be upregulated in several human cancer types. However, the clinical significance of Net-1 expression in NSCLC has remained elusive. The present study assessed Net-1 mRNA and protein levels by reverse-transcription quantitative polymerase chain reaction and western blot analysis of 64 cases of NSCLC as well as their adjacent normal tissues. Furthermore, Net-1 protein expression in tumor tissues derived from clinically annotated NSCLC cases at stages I-III was detected by immunohistochemical staining. The results showed that Net-1 mRNA and protein levels in NSCLC tissues were significantly elevated compared with those in their corresponding non-tumor tissues. In addition, Net-1 expression was strongly associated with the patients' pathological characteristics, including clinical stage, lymph node metastasis, distant metastasis and differentiation degree (P