Identification of soil micro‐habitats for growth, death and survival of a bacterium, γ‐1,2,3,4,5,6‐hexachlorocyclohexane‐assimilating Sphingomonas paucimobilis, by fractionation of soil
Identification of soil micro‐habitats for growth, death and survival of a bacterium, γ‐1,2,3,4,5,6‐hexachlorocyclohexane‐assimilating Sphingomonas paucimobilis, by fractionation of soil
复制标题
通过土壤分级鉴定细菌（γ-1,2,3,4,5,6-六氯环己烷同化少动鞘氨醇单胞菌）生长、死亡和生存的土壤微生境
DOI：
10.1111/j.1574-6941.1992.tb01650.x
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发表时间：
1992
影响因子：
2.1
通讯作者：
S. Matsumoto
中科院分区：
文献类型：
--
作者：
M. Nishiyama;K. Senoo;H. Wada;S. Matsumoto
土壤细菌少见鞘氨醇单胞菌是目前已知的唯一能好氧同化六六六(γ-1，2，3，4，5，6-γ)的细菌。自1973年以来，土生土长的同化γ-六氯环己烷的少见乳杆菌存活在每年使用γ-六氯环己烷的土壤中。相反，γ-六氯环己烷同化的少见葡萄球菌SS86菌株在对照土壤中接种后不能存活，但在γ-六氯环己烷的存在下可以繁殖。通过土壤分级鉴定了土壤中原生或接种γ-六氯环己烷同化少见乳链球菌的微生境。当土壤中加入γ-六氯环己烷时，本土的γ-六氯环己烷同化少见乳链球菌生长。生长中的土生细菌大多分布在土壤团聚体间孔隙的小于0.025 mm的细小颗粒中，随后死亡。长期存活的细菌主要集中在大于0.025 mm的土壤团聚体中。接种γ-六氯环己烷同化菌株SS86后，细菌定位于团聚体内，死亡速度较快。因此，细菌与土壤团聚体的结合可能与同化γ-六氯环己烷的少见链霉菌的长期生存有关。
A soil bacterium, Sphingomonas paucimobilis, is known to be the only bacterium which can aerobically assimilate γ-1,2,3,4,5,6-hexachlorocyclohexane (γ-HCH). Indigenous γ-HCH-assimilating S. paucimobilis survives in the soil where γ-HCH has been annually applied since 1973. In contrast, γ-HCH-assimilating S. paucimobilis strain SS86 cannot survive when inoculated into the control soil, although it can multiply in the presence of γ-HCH. Micro-habitats of γ-HCH-assimilating S. paucimobilis indigenous or inoculated into the soils were identified by fractionation of the soils. When γ-HCH was added to the soil, indigenous γ-HCH-assimilating S. paucimobilis grew. Most of the growing indigenous bacteria were found in fractions smaller than 0.025 mm which corresponded to soil inter-aggregate pores, and died afterwards. However, the indegenous bacteria which survived for a long period were found mainly in fractions larger than 0.025 mm which contained soil aggregates. When γ-HCH-assimilating strain SS86 was inoculated, the bacteria were located in inter-aggregate pores and died quickly. Consequently, association of the bacteria with soil aggregates was suggested to be related to the long-term survival of γ-HCH-assimilating S. paucimobilis.