Distribution of RAB5-positive multivesicular endosomes and the trans-Golgi network in root meristematic cells of Arabidopsis thaliana
Distribution of RAB5-positive multivesicular endosomes and the trans-Golgi network in root meristematic cells of Arabidopsis thaliana
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DOI：
10.5511/plantbiotechnology.16.0218a
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发表时间：
2016-09-01
影响因子：
1.6
通讯作者：
Nakano, Akihiko
中科院分区：
文献类型：
--
作者：
Ito, Emi;Uemura, Tomohiro;Nakano, Akihiko
在植物细胞中，trans-Golgi网络（TGN）被认为是早期的内吞区室，而RAB 5定位的多泡内体（MVEs）则是晚期的内吞区室。陆地植物和某些绿色藻类物种除了具有动物RAB 5的直系同源物（拟南芥中的RHA 1/RABF 2a和ARA 7/RABF 2b）之外，还具有植物特有的RAB 5同源物（拟南芥中的ARA 6/RABF 1）。thaliana），并且这两个RAB 5成员存在于MVE的基本上重叠但不同的亚群中。一些研究表明，TGN和MVE密切相关;然而，与TGN相关的两个RAB 5组的分布仍然难以捉摸。本研究定量分析表明，ARA 6和ARA 7与TGN密切相关，并且在拟南芥根表皮细胞中，ARA 6和ARA 7的亚群与TGN重叠。thaliana.
In plant cells, the trans-Golgi network (TGN) is known to act as the early endocytic compartment, whereas RAB5-localizing multivesicular endosomes (MVEs) act as the later compartment. Land plants and certain green algal species possess plant-unique RAB5 homologs (ARA6/RABF1 in Arabidopsis thaliana) in addition to the orthologs of animal RAB5 (RHA1/RABF2a and ARA7/RABF2b in A. thaliana), and these two RAB5 members reside in substantially overlapping but different subpopulations of MVEs. Several studies indicate that the TGN and MVEs are closely related; however, the distribution of the two RAB5 groups in relation to the TGN remains elusive. Here, we quantitatively showed that ARA6 and ARA7 are closely associated with the TGN, and the subpopulation of ARA6 and ARA7 overlaps with the TGN in the root epidermal cells of A. thaliana.