Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2
Environmental regulation of reproductive phase change in Agaricus bisporus by 1-octen-3-ol, temperature and CO2
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DOI：
10.1016/j.fgb.2013.01.001
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发表时间：
2013-06-01
影响因子：
3
通讯作者：
Burton, Kerry S.
中科院分区：
文献类型：
--
作者：
Eastwood, Daniel C.;Herman, Bram;Burton, Kerry S.
双孢蘑菇从营养菌丝体到开始结实的生殖阶段变化在很大程度上受环境条件感知的调控。提出了一个模型，其中三个独立的环境因素施加控制在不同阶段的生殖发育过程的变化。八碳挥发性1-辛烯-3-醇控制营养菌丝到多细胞节的早期分化;温度降低对后期原基的分化至关重要;二氧化碳水平对子实体发育的数量施加定量控制。利用启动特异性基因芯片分析了生殖阶段变化过程中转录组的变化，并对新发表的A。利用双孢酵母基因组分析差异表达基因的启动子区。我们的研究表明，有早期和晚期的启动反应有关的八个碳挥发物和温度分别感应。一个子集的45个基因的转录调控生殖阶段的变化，表现出一系列的功能，包括细胞结构，氮和碳代谢，传感和信号。三个基因簇连接增加转录与发育阶段。启动子区域的分析显示簇特异性保守的图案指示协调调节转录。(C)2013 Elsevier Inc. All rights reserved.
Reproductive phase change from vegetative mycelium to the initiation of fruiting in Agaricus bisporus is regulated in large part by the sensing of environmental conditions. A model is proposed in which three separate environmental factors exert control at different stages of the reproductive developmental process change. The eight carbon volatile 1-octen-3-ol controls the early differentiation from vegetative hyphae to multicellular knots; temperature reduction is essential for the later differentiation of primodia; and carbon dioxide level exerts quantitative control on the number of fruiting bodies developed. Analysis of transcriptomic changes during the reproductive phase change was carried out with initiation-specific microarrays, and the newly published A. bisporus genome was used to analyse the promoter regions of differentially regulated genes. Our studies have shown there to be both early and late initiation responses relating to sensing of eight carbon volatiles and temperature respectively. A subset of 45 genes was transcriptionally regulated during the reproductive phase change which exhibited a range of functions including cell structure, nitrogen and carbon metabolism, and sensing and signalling. Three gene clusters linking increased transcription with developmental stage were identified. Analysis of promoter regions revealed cluster-specific conserved motifs indicative of co-ordinated regulation of transcription. (C) 2013 Elsevier Inc. All rights reserved.