Characterization of smooth muscle-like cells in circulating human peripheral blood
Characterization of smooth muscle-like cells in circulating human peripheral blood
复制标题
DOI：
10.1016/j.atherosclerosis.2005.09.014
复制
发表时间：
2006-08-01
期刊：
影响因子：
5.3
通讯作者：
Libby, Peter
中科院分区：
文献类型：
--
作者：
Sugiyama, Seigo;Kugiyama, Kiyotaka;Libby, Peter
平滑肌细胞在人类血管疾病中起重要作用。几条证据表明，循环平滑肌前体细胞有助于动物模型中的内膜增生。我们从人外周血单核细胞（PBMC）中获得了表达α-平滑肌肌动蛋白（α-SMA）的大梭形细胞，在此表示为“平滑肌样细胞”（SMLC）。来源于人PBMC的SMLC在早期培养过程中容易增殖并表达促炎基因。长期培养后，SMLC可收缩并表达平滑肌细胞特征性标志物。我们发现外周血单个核细胞表达α-平滑肌肌动蛋白在循环血液中携带CD 14和CD 105。分选后的CD 14/CD 105双阳性PBMC可分化为SMLC。与无冠状动脉疾病的患者相比，冠状动脉疾病患者携带CD 14-CD 105的PBMC的数量显著增加。这些结果支持了平滑肌前体细胞存在于人循环血液中的新概念，并可能有助于血管疾病的发病机制。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Smooth muscle cells play an important role in human vascular diseases. Several lines of evidence demonstrate that circulating smooth muscle precursor cells contribute to intimal hyperplasia in animal models. We obtained large spindle cells expressing alpha-smooth muscle actin (alpha-SMA), denoted here as "smooth muscle-like cells" (SMLC), from human peripheral blood mononuclear cells (PBMC). SMLC derived from human PBMC proliferated readily and expressed pro-inflammatory genes during early culture. After long-term culture, SMLC could contract and express characteristic smooth muscle cell markers. We found peripheral blood mononuclear cell expressing alpha-smooth muscle actin in the circulating blood that bore CD14 and CD105. Sorted CD14/CD105 double-positive PBMC could differentiate into SMLC. The number of CD14-CD105-bearing PBMC increased significantly in patients with coronary artery disease compared to patients without coronary artery disease. These results support the novel concept that smooth muscle precursor cells exist in circulating human blood and may contribute to the pathogenesis of vascular diseases. (c) 2005 Elsevier Ireland Ltd. All rights reserved.