Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas chromatography-mass spectrometry -: Application to polytraumatized patients
Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas chromatography-mass spectrometry -: Application to polytraumatized patients
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DOI：
10.1016/j.jchromb.2008.07.037
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发表时间：
2008-09-01
影响因子：
3
通讯作者：
Durand, Thierry
中科院分区：
文献类型：
--
作者：
Mas, Emilie;Michel, Francoise;Durand, Thierry
异前列腺素是由多不饱和脂肪酸通过非酶自由基催化机制氧化而产生的前列腺素异构体家族。f -2异前列腺素（F-2-IsoPs）的定量提供了氧化应激的良好指标，并允许无创评估体内脂质过氧化。由于在m/z 573共洗脱液中优先使用d(4)-15-F-2t-IsoP的“干扰峰”，我们提出了一种新的GC-NICI-MS方法，以4(RS)- f -4t-神经前列腺素作为内标来定量尿液F-2-IsoPs。该方法被用于定量健康志愿者和多重创伤患者尿F-2-IsoPs排泄。我们的研究结果显示，与健康志愿者相比，多创伤患者尿F-2-IsoPs显著增加（p < 0.0001）（肌酐4.73 +/- 2.75 ng/mg vs. 0.811 +/- 0.359 ng/mg）。(c) 2008 Elsevier B.V.版权所有
Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F-2-isoprostanes (F-2-IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo. Since "interferences peaks" at m/z 573 co-elute with d(4)-15-F-2t-IsoP preferentially used, we propose a new GC-NICI-MS approach to quantify urinary F-2-IsoPs by using 4(RS)-F-4t-neuroprostane as the internal standard. This method was applied to quantify urinary F-2-IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase (p < 0.0001) in urinary F-2-IsoPs in polytraumatized patients compared with healthy volunteers (4.73 +/- 2.75 ng/mg vs. 0.811 +/- 0.359 ng/mg creatinine). (c) 2008 Elsevier B.V. All rights reserved.