Saccharomyces cerevisiae Sin3p facilitates DNA double-strand break repair
Saccharomyces cerevisiae Sin3p facilitates DNA double-strand break repair
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DOI：
10.1073/pnas.0304797101
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发表时间：
2004-02-10
影响因子：
11.1
通讯作者：
Jackson, SP
中科院分区：
文献类型：
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作者：
Jazayeri, A;McAinsh, AD;Jackson, SP
真核细胞中DNA双链断裂的修复主要有两条途径：同源重组和非同源末端连接。由于真核生物基因组包装在染色质中，这些途径可能需要染色质结构的调节。实现这一点的一种方法是通过组蛋白N端的赖氨酸残基的乙酰化。在这里，我们证明了酿酒酵母主要的组蛋白脱乙酰基酶复合体之一的组分Sin3p和Rpd3p是有效的非同源末端连接所必需的。我们还表明，组蛋白H4的赖氨酸16在染色体DNA双链断裂附近以Sin3p依赖的方式发生脱乙酰化。综上所述，这些结果定义了Sin3p/Rpd3p复合体在DNA修复调节中的作用。
There are two main pathways in eukaryotic cells for the repair of DNA double-strand breaks: homologous recombination and nonhomologous end joining. Because eukaryotic genomes are packaged in chromatin, these pathways are likely to require the modulation of chromatin structure. One way to achieve this is by the acetylation of lysine residues on the N-terminal tails of histones. Here we demonstrate that Sin3p and Rpd3p, components of one of the predominant histone deacetylase complexes of Saccharomyces cerevisiae, are required for efficient nonhomologous end joining. We also show that lysine 16 of histone H4 becomes deacetylated in the proximity of a chromosomal DNA double-strand break in a Sin3p-dependent manner. Taken together, these results define a role for the Sin3p/Rpd3p complex in the modulation of DNA repair.