Structure of a Polycomb response element and in vitro binding of Polycomb group complexes containing GAGA factor
Structure of a Polycomb response element and in vitro binding of Polycomb group complexes containing GAGA factor
复制标题
DOI：
10.1128/mcb.20.9.3187-3197.2000
复制
发表时间：
2000-05-01
影响因子：
5.3
通讯作者：
Pirrotta, V
中科院分区：
文献类型：
--
作者：
Horard, B;Tatout, C;Pirrotta, V
多梳反应元件(PreS)是调节同源异型基因和其他基因沉默的调节位点。果蝇超双胸腺基因BxD Pre区可分为100-200bp的亚片段，在体内保留不同程度的Pre活性。在体外，胚胎核抽提物与这些片段形成含有聚梳基(PcG)蛋白的复合体。PCG与某些片段的结合依赖于GagA因子的共同序列。其他片段缺乏GagA结合部位，但在体外仍能结合PcG复合体。我们证明了Gaga因子是至少某些类型的PcG络合物的组成部分，并且可能参与了PcG络合物在PreS的组装。
Polycomb response elements (PREs) are regulatory sites that mediate the silencing of homeotic and other genes. The bxd PRE region from the Drosophila Ultrabithorax gene can be subdivided into subfragments of 100 to 200 bp that retain different degrees of PRE activity in vivo. In vitro, embryonic nuclear extracts form complexes containing Polycomb group (PcG) proteins with these fragments. PcG binding to some fragments is dependent on consensus sequences for the GAGA factor. Other fragments lack GAGA binding sites but can still bind PcG complexes in vitro. We show that the GAGA factor is a component of at least some types of PcG complexes and may participate in the assembly of PcG complexes at PREs.