OsERF101, an ERF family transcription factor, regulates drought stress response in reproductive tissues
OsERF101, an ERF family transcription factor, regulates drought stress response in reproductive tissues
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OsERF101 是一种 ERF 家族转录因子，调节生殖组织的干旱应激反应
DOI：
10.1007/s11103-018-0762-5
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发表时间：
2018-09-01
影响因子：
5.1
通讯作者：
Ge, Xiaochun
中科院分区：
文献类型：
--
作者：
Jin, Yue;Pan, Weiyang;Ge, Xiaochun
关键信息ERF转录因子OsERF101主要在水稻生殖组织中表达，在干旱胁迫下提高水稻结实率方面发挥着重要作用。摘要干旱对植物营养和生殖发育过程的累积伤害导致粮食产量下降。然而，参与这些过程的基因仍然不完全清楚。在本研究中，我们发现了一个名为OsERF101的基因，它在生殖和营养阶段对脱水胁迫的适应性反应中起着重要的正向调节作用。该基因编码APETALA2/乙烯反应元件结合蛋白(AP2/EREBP)家族成员。在正常生长条件下，OsERF101主要在花中表达，特别是在绒毡层和小孢子中。干旱、聚乙二醇6000和脱落酸(ABA)均可诱导叶片发生褐变。在营养生长期，过表达OsERF101的植株比对照植株对PEG6000引起的渗透胁迫具有更强的抗性。在生殖期干旱胁迫下，它们的成活率和结实率也高于野生型。进一步的生理分析表明，高表达品系的花粉育性有所提高，而基因敲除突变体和RNAi品系在繁殖期表现出花粉育性降低和耐旱性降低。OsERF101高表达植株中的脯氨酸含量和过氧化物酶活性的增加可能有助于提高植物的抗旱性。此外，OsERF101过表达植株表现出对ABA敏感的表型，其中ABA反应基因RD22、LEA3和PODS的表达水平上调。综上所述，我们的结果表明，OsERF101是一个在营养和生殖阶段调节脱水反应的基因。
Key messageAn ERF transcription factorOsERF101is predominantly expressed in rice reproductive tissues and plays an important role in improving rice seed setting rate under drought stress.AbstractDrought reduces grain yield due to the cumulative damage effects to plant vegetative and reproductive developmental processes. However, the genes involved in these processes are still not completely understood. In this study, we identified a gene namedOsERF101as an important positive regulator in the adaptive responses to dehydration stress during the reproductive and vegetative stages. This gene encodes a member of APETALA2/Ethylene-Responsive Element Binding Protein (AP2/EREBP) family.OsERF101was predominantly expressed in flowers, particularly in the tapetum and microspores under normal growth conditions. It was induced by drought, PEG6000 and abscisic acid (ABA) in leaves. During the vegetative stage,OsERF101-overexpression plants were more resistant to osmotic stress caused by PEG6000 compared to the control plants. They also had higher survival and seed setting rates than wild type when subjected to reproductive-stage drought stress. Further physiological analysis revealed that the pollen fertility was improved in the overexpression lines, while the knockout mutant and RNAi lines showed reduced pollen fertility and compromised drought tolerance during the reproductive stage. The increased proline content and peroxidase activity inOsERF101-overexpression plants might contribute to the improved drought-tolerance of plants. In addition,OsERF101-overexpression plants displayed ABA susceptible phenotype, in which the expression levels of ABA-responsive genesRD22, LEA3, andPODswere up-regulated. Taken together, our results indicate thatOsERF101is a gene that regulates dehydration responses during the vegetative and reproductive stages.