The melanoma antigen gp75 is the human homologue of the mouse b (brown) locus gene product.
The melanoma antigen gp75 is the human homologue of the mouse b (brown) locus gene product.
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黑色素瘤抗原GP75是小鼠B（棕色）基因座基因产物的人类同源物。
DOI：
10.1084/jem.171.4.1375
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发表时间：
1990-04-01
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gp75 抗原是一种丰富的细胞内糖蛋白，在人类色素细胞和黑色素瘤的黑色素体中表达。转移性黑色素瘤患者血清中的 IgG 抗体已被证明可以免疫沉淀 gp75，这表明可以打破针对 gp75 的免疫耐受。使用特异性识别 gp75 的小鼠单克隆抗体 TA99 来分离和纯化抗原。从纯化的 gp75 多肽中确定了三种内部肽的氨基酸序列。通过用基于这些肽序列的寡核苷酸筛选来分离cDNA克隆。 gp75 肽和 cDNA 分别与映射到 b（棕色）基因座的小鼠基因的衍生氨基酸和核苷酸序列具有大约 90% 的同一性。棕色基因座决定小鼠的毛色，表明 gp75 调节或影响黑色素合成的类型。
The gp75 antigen is an abundant intracellular glycoprotein expressed in melanosomes of human pigmented melanocytes and melanomas. IgG antibodies in sera of a patient with metastatic melanoma have been shown to immunoprecipitate gp75, suggesting that immunological tolerance against gp75 can be broken. The mouse mAb TA99, which specifically recognizes gp75, was used to isolate and purify the antigen. Amino acid sequences of three internal peptides were determined from the purified gp75 polypeptide. cDNA clones were isolated by screening with oligonucleotides based on these peptide sequences. The gp75 peptides and cDNA had approximately 90% identity with, respectively, the derived amino acid and nucleotide sequences of a mouse gene that maps to the b (brown) locus. The brown locus determines coat color in the mouse, suggesting that gp75 regulates or influences the type of melanin synthesized.