Simultaneous co-infection with swine influenza A and porcine reproductive and respiratory syndrome viruses potentiates adaptive immune responses.
Simultaneous co-infection with swine influenza A and porcine reproductive and respiratory syndrome viruses potentiates adaptive immune responses.
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DOI：
10.3389/fimmu.2023.1192604
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发表时间：
2023
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
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作者：
猪呼吸道疾病是多因素引起的，最常见的是病原体混合感染。主要贡献者包括猪流感A（swIAV）和猪繁殖与呼吸综合征（PRRSV）病毒。这两种病毒的实验性共感染研究表明，临床结果可能会恶化，但先天性和适应性免疫反应如何促进发病机制和病原体控制尚未得到彻底评估。我们研究了猪实验性同时感染swIAV H3 N2和PRRSV-2后的免疫应答。我们的研究结果表明，临床疾病并没有显着加剧，和swIAV H3 N2病毒载量减少，在肺部的共同感染的动物。PRRSV-2/swIAV H3 N2共感染不会损害病毒特异性适应性免疫应答的发展。增强了swIAV H3 N2特异性IgG血清滴度和血液中的PRRSV-2特异性CD 8 β+ T细胞应答。与单一感染组相比，在PRRSV-2/swIAV H3 N2共感染动物中发现血液和肺洗液中多功能CD 8 β+ T细胞亚群的比例更高。我们的研究结果提供的证据表明，系统和局部宿主免疫反应不会受到同时swIAV H3 N2/PRRSV-2共感染的负面影响，提出了有关疾病调节机制的问题。
Porcine respiratory disease is multifactorial and most commonly involves pathogen co-infections. Major contributors include swine influenza A (swIAV) and porcine reproductive and respiratory syndrome (PRRSV) viruses. Experimental co-infection studies with these two viruses have shown that clinical outcomes can be exacerbated, but how innate and adaptive immune responses contribute to pathogenesis and pathogen control has not been thoroughly evaluated. We investigated immune responses following experimental simultaneous co-infection of pigs with swIAV H3N2 and PRRSV-2. Our results indicated that clinical disease was not significantly exacerbated, and swIAV H3N2 viral load was reduced in the lung of the co-infected animals. PRRSV-2/swIAV H3N2 co-infection did not impair the development of virus-specific adaptive immune responses. swIAV H3N2-specific IgG serum titers and PRRSV-2-specific CD8β+ T-cell responses in blood were enhanced. Higher proportions of polyfunctional CD8β+ T-cell subset in both blood and lung washes were found in PRRSV-2/swIAV H3N2 co-infected animals compared to the single-infected groups. Our findings provide evidence that systemic and local host immune responses are not negatively affected by simultaneous swIAV H3N2/PRRSV-2 co-infection, raising questions as to the mechanisms involved in disease modulation.
DOI：
10.3390/pathogens9110934
发表时间：
2020-11-11
期刊：
Pathogens (Basel, Switzerland)
影响因子：
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作者：
Fleming DS;Miller LC;Tian Y;Li Y;Ma W;Sang Y
通讯作者：
Sang Y