Variation at the insulin-like growth factor 1 gene and its association with body weight traits in the chicken
Variation at the insulin-like growth factor 1 gene and its association with body weight traits in the chicken
复制标题
鸡类胰岛素生长因子 1 基因的变异及其与体重特征的关联。
DOI：
10.1111/j.1439-0388.2008.00739.x
复制
发表时间：
2008-08-01
影响因子：
2.6
通讯作者：
Li, H.
中科院分区：
文献类型：
--
作者：
Bian, L. H.;Wang, S. Z.;Li, H.
The insulin-like growth factor 1 (IGF1) is essential for normal embryonic and postnatal growth in mammals. In this study, a total of 342 F(2) individuals, derived from Broiler crossing to Baier layer (Northeast Agricultural University Resource Population, NEAURP), were used to investigate the associations of haplotypes in the chicken IGF1 (cIGF1) gene with body weight traits. Primers for the 5'-flanking, exon 3 and 3'-flanking regions of cIGF1 were designed according to chicken genome database. Single nucleotide polymorphisms (SNPs) between parental lines were detected by sequencing, and PCR restriction fragment length polymorphism (PCR-RFLP) and PCR single-stranded-conformation polymorphism (PCR-SSCP) methods were used to genotype the SNPs in the population. Haplotypes were constructed with the three SNPs detected. The association analysis showed that haplotypes based on three cIGF1 polymorphisms (c.-366A>C, c.528G>A and c.*1024C>T) were associated with body weight traits, suggesting that cIGF1 or a tightly linked gene had effects on body weight in the chicken.
