Seasonal and spatial variability of free-living bacterial community composition along an estuarine gradient (Ria de Aveiro, Portugal)
Seasonal and spatial variability of free-living bacterial community composition along an estuarine gradient (Ria de Aveiro, Portugal)
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DOI：
10.1016/j.ecss.2006.01.015
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发表时间：
2006-06-01
影响因子：
2.8
通讯作者：
Correia, Antonio
中科院分区：
文献类型：
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作者：
Henriques, Isabel S.;Alves, Artur;Correia, Antonio
我们使用16 S rDNA PCR-变性梯度凝胶电泳（DGGE）研究了葡萄牙里亚德阿韦罗河口自由生活细菌群落的动态。样品收集沿着盐度梯度在2003年4月，7月和10月和2004年1月。DGGE剖面分析表明，该社区内的组成变化发生在半咸水和淡水部分之间。这些变化在4月、10月和1月采集的样本中尤为明显。不太明显的变化也检测到沿着盐度梯度在海洋和半咸水部分。在这个河口的微生物群落季节性驱动的变化也发生。典范对应分析（CCA）显示，盐度和温度占浮游细菌群落组成的变化（26%）的显着金额。通过直接测序或通过克隆和测序DGGE条带来鉴定优势的突变类型。DNA序列分析表明，河口海水微咸水段的优势菌群由拟杆菌门、α-变形菌门和γ-变形菌门转变为淡水段的拟杆菌门、β-变形菌门、δ-变形菌门和ε-变形菌门。(c)2006爱思唯尔有限公司保留所有权利。
We examined the dynamics of the free-living bacterial community in the Ria de Aveiro estuary (Portugal) using 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE). Samples were collected along the salinity gradient in April, July and October 2003 and January 2004. Analysis of DGGE profiles indicated that the compositional shifts within this community occurred between the brackish and freshwater sections. Those shifts were particularly evident in samples collected in April, October and January. Less pronounced changes were also detected along the salinity gradient in the marine and brackish sections. Seasonally driven changes in microbial community in this estuary also occur. Canonical correspondence analysis (CCA) revealed that salinity and temperature accounted for a significant amount of the variability in the bacterioplankton community composition (26%). Dominant phylotypes were identified either by direct sequencing or by cloning and sequencing DGGE bands. Analysis of DNA sequences revealed that the dominant bacterial groups changed from Bacteroidetes, Alpha-proteobacteria and Gamma-proteobacteria in the marine-brackish section to Bacteroidetes, Beta-proteobacteria, Delta-proteobacteria and Epsilon-proteobacteria in the freshwater section of the estuary. (c) 2006 Elsevier Ltd. All rights reserved.