Localization of cyclic adenosine monophosphate in the teleost retina: effects of dopamine and prolonged darkness.
Localization of cyclic adenosine monophosphate in the teleost retina: effects of dopamine and prolonged darkness.
复制标题
环磷酸腺苷在硬骨鱼视网膜中的定位：多巴胺和长时间黑暗的影响。
DOI：
10.1016/0006-8993(89)91597-7
复制
发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Dowling,JE
中科院分区：
文献类型：
--
作者：
Young,LH;Dowling,JE
用免疫组织化学和放射自显影方法对白色鲈鱼视网膜内的环磷酸腺苷（cAMP）进行了定位。在暴露于多巴胺或长时间黑暗后，通过免疫组织化学在内核层的远端部分（即在水平细胞中）观察到cAMP染色。在暴露于多巴胺后，通过放射自显影术也在许多水平细胞中观察到cAMP水平增加。最后，增加水平的cAMP染色，观察到与多巴胺孵育后，在所有类型的锥相关的水平细胞，已被分离和保持在培养物中。
Localization of cyclic adenosine monophosphate (cAMP) in the white perch retina was carried out with immunohistochemical and autoradiographic methods. Following exposure to dopamine or prolonged darkness, cAMP staining was observed by immunohistochemistry in the distal part of the inner nuclear layer, i.e. in the horizontal cells. After exposure to dopamine, increased levels of cAMP were also observed by autoradiography in many horizontal cells. Finally, increased levels of cAMP staining were observed immunohistochemically following incubation with dopamine in all types of cone-related horizontal cells that had been isolated and maintained in culture.