The importance of calcium ions for the regulation of guanosine 3':5'-cyclic monophosphage levels.
The importance of calcium ions for the regulation of guanosine 3':5'-cyclic monophosphage levels.
复制标题
钙离子对于调节鸟苷 3:5-环状单磷酸噬菌体水平的重要性。
DOI：
10.1073/pnas.70.12.3889
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发表时间：
1973
影响因子：
11.1
通讯作者：
E. Sutherland
中科院分区：
文献类型：
--
作者：
G. Schultz;J. Hardman;K. Schultz;C. Baird;E. Sutherland
乙酰胆碱（10-1000 muM）或125 mM KCl可使大鼠肾管中鸟苷3′：5′-环单磷酸（cyclic GMP）水平增加2 ~ 3倍，而腺苷3′：5′-环单磷酸（cyclic AMP）水平无变化。在没有Ca（++）的情况下孵育30分钟后，环GMP控制水平下降了85%，并且不受乙酰胆碱或KCl的影响。Ca(++) （1.8 mM）在Ca（++）缺失的组织中持续3分钟，部分恢复了基础环GMP水平和乙酰胆碱和KCl的作用。添加Sr(++) （3.6 mM）或Ba(++)（1.8或10 mM）也可引起Ca（++）缺失组织中基础环GMP的增加。在这些条件下，环AMP水平没有显著变化。磷酸二酯酶抑制剂1-甲基-3-异丁基黄嘌呤（0.1 mM）的加入使导管内环AMP的量增加约50%，环GMP的量增加约2倍。后一种效应也取决于Ca（++）的存在。1-甲基-3-异丁基黄嘌呤（0.1 mM）可提高大鼠颌下腺切片中环GMP和环AMP的水平。如果加入甲基异丁基黄嘌呤，甲基胆碱增加了环GMP。Ca（++）的遗漏使环GMP控制水平和甲基异丁基黄嘌呤的作用保持不变，但甲基胆碱的作用被消除。这些发现表明钙离子对这些组织中环GMP水平的控制很重要。
Guanosine 3':5'-cyclic monosphosphate (cyclic GMP) levels in the ductus deferens of the rat were increased 2- to 3-fold by acetylcholine (10-1000 muM) or by 125 mM KCl, while adenosine 3':5'-cyclic monophosphate (cyclic AMP) levels were not changed. After incubation for 30 min in the absence of Ca(++), cyclic GMP control levels were decreased by 85% and were not affected by acetylcholine or KCl. The readdition of Ca(++) (1.8 mM) for 3 min to Ca(++)-deprived tissue partially restored basal cyclic GMP levels and the effects of acetylcholine and KCl. The addition of Sr(++) (3.6 mM) or of Ba(++) (1.8 or 10 mM) also caused an increase in basal cyclic GMP in Ca(++)-deprived tissue. Cyclic AMP levels were not significantly changed under any of these conditions. The addition of the phosphodiesterase inhibitor, 1-methyl-3-isobutylxanthine (0.1 mM), to ductus deferentes increased the amount of cyclic AMP about 50% and that of cyclic GMP about 2-fold. The later effect also depended on the presence of Ca(++). 1-Methyl-3-isobutylxanthine (0.1 mM) increased cyclic GMP and cyclic AMP levels in slices of rat submaxillary glands. Methacholine increased cyclic GMP if added in the presence of methyl isobutylxanthine. Cyclic GMP control levels and the effect of methyl isobutylxanthine were unchanged by Ca(++) omission, but the effect of methacholine was abolished.These findings indicate that calcium ions are important for the control of cyclic GMP levels in these tissues.