Dispersal patterns of endogenous bacteria among grass carp (Ctenopharyngodon idellus) guts
Dispersal patterns of endogenous bacteria among grass carp (Ctenopharyngodon idellus) guts
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DOI：
10.22092/ijfs.2018.114683
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发表时间：
2017-04
影响因子：
0.9
通讯作者：
J. Ni;Q. Yan;Y. H. Yu;H. H. Wu-H.;F. Chen
中科院分区：
文献类型：
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作者：
J. Ni;Q. Yan;Y. H. Yu;H. H. Wu-H.;F. Chen
脊椎动物内源性肠道微生物区系对宿主的营养、发育和健康起着至关重要的作用，其形成和调控已被广泛研究。尽管微生物区系对宿主健康很重要，但目前仍不清楚内源性肠道微生物在不同宿主个体中是在基因上是截然不同的，还是在基因上相互关联。本研究通过构建细菌16S rRNA基因克隆文库，研究了内源性肠道细菌在草鱼体内的扩散情况。结果表明，草鱼肠道中的细菌可分为以下两类：a-私有操作分类单位(OTUS)，包括溶血杆菌、詹达气单胞菌、弗氏柠檬酸杆菌、木糖嗜酸杆菌和类杆菌；b-共享的OTUS，包括霍乱弧菌、志贺氏菌和巴氏杆菌。本研究结果为评估内源性肠道细菌，特别是致病菌的传播机制提供了有价值的信息。然而，不同方式的细菌在草鱼肠道中扩散的机制仍需进一步研究。
The formation and regulation of vertebrate endogenous intestinal microbiota has been widely studied as the microbiota plays a crucial role in the host nutrition, development, and health. Despite the importance of microbiota for host health, it is still unclear whether the endogenous intestinal microorganisms are genetically distinct or whether they are genetically related with each other in different host individuals. In the present study, the dispersal situation of the endogenous intestinal bacteria in grass carp was investigated by constructing bacterial 16S rRNA gene clone libraries. The results indicate that the bacteria harbored in the grass carp gut could be separated into the following two groups: a- the private operational taxonomic units (OTUs), which include Cetobacterium somerae, Aeromonas jandaei, Citrobacter freundii, Achromobacter xylosoxidans and Bacteroides species; b- the shared OTUs, which include Vibrio cholerae, Plesiomonas shigelloides and Pasteurella speices. The results obtained in this investigation provide valuable information for assessing the mechanism of spread of the endogenous intestinal bacteria, especially the pathogenic ones. However, the mechanisms involved in different modes of bacterial dispersal in the grass carp gut still require further research.