Chemical composition and tyrosinase inhibitory activity of Cinnamomum cassia essential oil.
Chemical composition and tyrosinase inhibitory activity of Cinnamomum cassia essential oil.
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DOI：
10.1186/1999-3110-54-10
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发表时间：
2013-12
影响因子：
3.4
通讯作者：
Chou ST
中科院分区：
文献类型：
--
作者：
Chang CT;Chang WL;Hsu JC;Shih Y;Chou ST
从芳香植物中提取的精油具有重要的生物活性，并且在科学研究中变得越来越重要。从肉桂中提取的精油（CC-EO）具有多种功能特性，然而，关于酪氨酸酶抑制活性的信息很少。因此，本研究的目的是研究CC-EO的化学组成和酪氨酸酶抑制活性。气相色谱-质谱联用（GC-MS）分析表明，CC-EO的主要成分为肉桂醛（42.37%）和顺式-2-甲氧基肉桂酸（43.06%）。进一步评价了CC-EO及其主要成分对蘑菇酪氨酸酶的抑制活性。结果表明，CC-EO和肉桂醛对酪氨酸酶有抑制作用，IC 50分别为6.16 ± 0.04 mg/mL和4.04 ± 0.08 mg/mL。然而，顺式-2-甲氧基肉桂酸没有显示出任何抗酪氨酸酶活性。采用Lineweaver-Burk作图法和二次曲线法对缓蚀动力学进行了分析，结果表明CC-EO和肉桂醛为混合型缓蚀剂。计算出CC-EO和肉桂醛的抑制常数（Ki）分别为4.71 ± 0.09 mg/mL和2.38 ± 0.09 mg/mL。这些结果表明，CC-EO及其主要成分肉桂醛具有有效的抗酪氨酸酶活性，可能是皮肤美白剂的良好来源。本文的在线版本（doi：10.1186/1999-3110-54-10）包含补充材料，可供授权用户使用。
Essential oils extracted from aromatic plants exhibit important biological activities and have become increasingly important for scientific research. The essential oil extracted from Cinnamomum cassia Presl (CC-EO) has various functional properties, however, little information is available regarding the tyrosinase inhibitory activity. Therefore, the objectives of this study were to investigate the chemical composition and tyrosinase inhibitory activity of the CC-EO. cis-2-methoxycinnamic acid (43.06%) and cinnamaldehyde (42.37%) were found to be the two major components of the CC-EO identified by gas chromatography–mass spectrometry (GC-MS). The inhibitory activities of CC-EO and its major constituents were further evaluated against mushroom tyrosinase. The results showed that CC-EO and cinnamaldehyde exhibited anti-tyrosinase activities with IC50 values of 6.16 ± 0.04 mg/mL and 4.04 ± 0.08 mg/mL, respectively. However, cis-2-methoxycinnamic acid did not show any anti-tyrosinase activity. The inhibition kinetics were analyzed by Lineweaver-Burk plots and second replots, which revealed that CC-EO and cinnamaldehyde were mixed-type inhibitors. The inhibition constants (Ki) for CC-EO and cinnamaldehyde were calculated to be 4.71 ± 0.09 mg/mL and 2.38 ± 0.09 mg/mL, respectively. These results demonstrate that CC-EO and its major component, cinnamaldehyde, possess potent anti-tyrosinase activities and may be a good source for skin-whitening agents. The online version of this article (doi:10.1186/1999-3110-54-10) contains supplementary material, which is available to authorized users.